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1997 Fiscal Year Final Research Report Summary

Analysis of dynein function by use of mutants and in vitro motility assay systems

Research Project

Project/Area Number 07408034
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionUniversity of Tokyo

Principal Investigator

KAMIYA Ritsu  University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (10124314)

Co-Investigator(Kenkyū-buntansha) YOSHIMURA Kenjiro  University of Tokyo, Graduate School of Science, Research Associate, 大学院・理学系研究科, 助手 (10230806)
HIRONO Masafumi  University of Tokyo, Graduate School of Science, Lecturer, 大学院・理学系研究科, 講師 (10212177)
Project Period (FY) 1995 – 1997
Keywordsflagella / dynein / actin / genes / mutation / Chlamydomonas / cell motility / force production
Research Abstract

The basis for the beating of eukaryotic cilia and flagella is the sliding movement of microtubules driven by dynein, but how undulating movements are produced is not understood. To approach this problem, we carried out two lines of studies aimed at elucidation of the interaction between microtubules and various kinds of dynein. First, we tried to isolate and characterize Chlamydomonas mutants that lack specific dynein subspecies, and assess the property of the missing dyneins by analyzing the motility of the mutants. Second, we isolated specific dynein subspecies, and assayd their properties using in vitro motility systems. Major findings from these studies are as follows : (1) ida5, a mutant missing a subset of inner arm dyneins, was found to have a mutation in the structural gene of conventional actin, such that no conventional actin is produced in the cell. The discovery of this mutant will help to clarify the physiological role of actin in inner-arm dyneins. (2) The force generating properties of dyneins were found to differ greatly between inner and outer arm dyneins ; in particular, the inner arm is more important than the outer arm when axoneme is moving slowly, such as in the process of bend initiation. (3) The activity of dynein is regulated by nucleotides, such as ADP.(4) Outer arm dynein can be self-activated to produce force under various conditions, while inner arm dyneins cannot-they need the central pair and radial spokes for force generation. In short, this study has revealed a great functional diversity among different dyneins. This diversity appears to be important for the axonemal function.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Minoura, I.: "Strikingly different propulsive forces generated by different dynein deficient Chlamydomonas mutants in viscous media" Cell Motil.Cytoskeleton. 31. 130-139 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yagi, T.: "Novel mode of hyper-oscillation in the paralyzed axoneme of a Chlamydomonas mutant lacking the central-pair microtubules" Cell Motil.Cytoskeleton. 31. 207-214 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogawa, K.: "Interspecies conservation of outer arm dynein intermediate chain sequences defines two intermediate chain subclasses" Molec.Biol.Cell. 6. 685-696 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamiya, R.: "Exploring the function of inner and outer dynein arms with Chlamydomonasmutants" Cell Motil.Cytoskeleton. 32. 98-102 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kagami, O.: "Nonradioactive method for ATPaseassays" Methods Cell Biol.47. 147-150 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kagami, O.: "Separation of dynein species by high-pressure liquid chromatography" Methods Cell Biol.47. 487-489 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamiya, R.: "Strategies for isolation of flagellar motility and assembly mutants in Chlamydomonas" Methods Cell Biol.47. 541-544 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, S.: "Assembly and function of Chlamydomonas flagellar mastigonemes as probed with a monoclonal antibody" J.Cell Sci.109. 57-62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Omoto, C.K.: "Ability of paralyzed flagella mutants of Chlamydomonas to move" Cell Motil.Cytoskeleton. 33. 88-94 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugase, Y.: "Cloning and characterization of the actin-encoding gene og Chlamydomonasreinhardtii" Gene. 168. 117-121 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takada, S.: "Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer-doublet microtubules" Cell Motil.Cytoskeleton. 36. 68-75 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato-Minoura, T.: "Chlamydomonas inner-arm dynein mutant,ida5,has a mutation in an actin-encoding gene" J.Cell Biol.137. 649-656 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashibe, K.: "Induction of temporary beating in paralyzed flagella of Chlamydomonas mutants by application of external force" Cell Motil.Cytoskeleton. 37. 232-239 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wakabayashi, K.: "Ca2^+-dependent waveform conversion in the flagellar axoneme of Chlamydomonas mutants lacking the central-pair/radial spoke system" Cell Motil.Cytoskeleton. 38. 22-28 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minoura, I.and Kamiya, R.: "Strikingly different propulsive forces generated by different dynein deficient Chlamydomonas mutants in viscous media" Cell Motil.Cytoskeleton. 31. 130-139 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yagi, T.and Kamiya, R.: "A novel mode of hyper-oscillation in the paralyzed axoneme of a Chlamydomonas mutant lacking the central-pair microtubules." Cell Motil.Cytoskeleton. 31. 207-214 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa, K., Kamiya, R., Wilkerson, C.G., and Witman, G.B.: "Interspecies conservation of outer arm dynein intermediate chain sequences defines two intermediate chain subclasses" Molec.Biol.Cell. 6. 685-696 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamiya, R.: "Exploring the function of inner and outer dynein arms with Chalamydomonas mutants." Cell Motil.Cytoskeleton. 32. 98-102 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kagami, O.and Kamiya, R.: "Nonradioactive method for ATPase assays." Methods Cell Biol.47. 147-150 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kagami, O.and Kamiya, R.: "Separation of dynein species by high-pressure liquid chromatography." Methods Cell Biol.47. 487-489 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamiya, R.: "Strategies for isolation of flagellar motility and assembly mutants in Chlamydomonas" Methods Cell Biol.47. 541-544 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, S., Tanaka, G., Maeda, T., Kamiya, R., Matsunaga, T.and Nikaido, O.: "Assembly and function of Chlamydomonas flagellar mastigonemes as probed with a monoclonal antibody." J.Cell Sci.109. 57-62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugase, Y., Hirono, M., Kindle, K.L., and Kamiya, R.: "Cloning and characterization of the actin-encoding gene of Chlamydomonas reinhardtii." Gene 168. 117-121 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Omoto, C.K., Yagi, T., Kurimoto, E., and Kamiya, R.: "Ability of paralyzed flagella mutants of Chlamydomonas to move" Cell Motil.Cytoskeleton. 33. 88-94 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takada, S.and Kamiya, R.: "Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer doublet microtubules." Cell Motil.Cytoskeleton. 36. 68-75 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato-Minoura, T., Hirono, M., and Kamiya, R.: "Chlamydomonas inner-arm dynein mutant, ide5, has a mutation in an actin encoding gene." J.Cell Biol.137. 68-75 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashibe, K., Shingyoji, C.and Kamiya, R.: "Induction of temporary beating in paralyzed flagella of Chlamydomonas mutants by application of external force" Cell Motil.Cytoskeleton. 37. 232-239 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wakabayashi, K., Yagi, T.and Kamiya, R.: "Ca^<2+>-dependent waveform conversion in the flagellar axoneme of Chlamydomonas mutants lacking the central-pair/radial spoke system" Cell Motil.Cytoskeleton. 38. 22-28 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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