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1996 Fiscal Year Final Research Report Summary

Bioinorganic Chemical Study on Structure and Function of Cu-containing Nitrite Reductase

Research Project

Project/Area Number 07454177
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Inorganic chemistry
Research InstitutionOsaka University

Principal Investigator

SUZUKI Shinnichiro  Osaka University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (70116052)

Co-Investigator(Kenkyū-buntansha) KATAOKA Kunishige  Osaka University, Graduate School of Science, Rsearch Assistant, 大学院・理学研究科, 助手 (40252712)
YAMAGUCHI Kazuya  Osaka University, Graduate School of Science, Rsearch Assistant, 大学院・理学研究科, 助手 (80252550)
Project Period (FY) 1995 – 1996
Keywordsdenitrifying bacteria / nitrite reductase / type 1 Cu / type 2 Cu / azurin / blue copper protein / pulse radiolysis / intramolecular electron transfer
Research Abstract

The purpose of this project is to clarify the structure and function of Cu-containing nitrite reductases (NIRs) from Achromobacter cycloclastes IAM1013, Alcaligenes sp., and Achromobacter xylosoxidans GIFU strains.
1) NIRs from Achromobacter cycloclastes IAM1013, Alcaligenes sp., and Achromobacter xylosoxidans GIFU1051 contain two types of Cu (type 1 Cu and type 2 Cu). Type 2 Cu-depleted (T2D) NIRs were prepared by a chemical treatment. The present T2D enzymes have no type 2 Cu. 2) The spectroscopic contribution of the type 2 Cu site in the region of 300-800 nm is ascertained to be quite small by a comparison between native and T2D NIRs. 3) According to the NIR assay system, T2D NIRs show the enzymatic activities, although they are lower than those of the native enzymes. 4) The intramolecular electron transfer (ET) process obeys first-order kinetics and observed rate constants are 1,400-1,900s^<-1> (t_<1/2>=ca.0.5 msec) at pH7.0. In the presence of nitrite, the ET process also obeys first-order kinetics with rate constants decreased by factors of 1/12-1/2 at the same pH.Nitrite would shift the redox potential of the type 2 Cu by coordination, and hence the intramolecular ET rate constant is decreased. 5) NIRs from four GIFU strains (GIFU 543, GIFU 1048, GIFU 1055, and GIFU 1764) are quite similar to those from Alcaligenes sp.and GIFU1051. These blue NIRs show the absorption maxima at 594 nm. 6) The strains of GIFU 543 and GIFU 1048 contain two kinds of azurin (azurin-1 and azurin-2) as well as Alcaligenes sp.and GIFU1051 strains, whereas the strains of GIFU 1055 and GIFU 1764 produce azurin-1 only.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] S. Suzuki, Deligeer, K. Yamaguchi, K. Kataoka et al.: "Spectroscopic Characterization and Intramolecular Electron Transfer Processes of Native and Type 2 Cu-depleted Nitrite Reductases" J. Biol. Inorg. Chem.2 (2) (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Maritano, A. Marchesini, S. Suzuki: "Spectroscopic Characterization of Native and Co (II)-substituted Zucchini Mavicyanin" J. Biol. Inorg. Chem.2 (2) (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Yoshimura, S. Fujii, H. Kamada, K. Yamaguchi et al.: "Spectroscopic Characterization of Nitrosylheme in Nitric Oxide Complexes of Ferric and Ferrous Cytochrome c' from Photosynthetic Bacteria" Biochim. Biophys. Acta. 1292. 39-46 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Choi, R. Matsuzaki, S. Suzuki, K. Tanizawa: "Role of Conserved Asn-Tyr-Asp-Tyr Sequence in Bacterial Copper/2, 4, 5-Trihydroxy-phenylalanyl Quinone-containing Histamine Oxidase" J. Biol. Chem.271. 22598-22603 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Sakurai, F. Nose, T. Fujiki,S. Suzuki: "Reduction and Oxidation Processes of Blue Copper Proteins, Azurin, Pseudoazurin, Umecyanin, Stellacyanin, Plantacyanin, and Plastocyanin Approached by Cyclic and Potential Step Voltammetries" Bull. Chem. Soc. Jpn.69. 2855-2862 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Kohzuma, S. Suzuki: "Spectroscopic and Electrochemical Studies on the Four-α-helices Bundle Cytochrome'c" Mol. Cryst. Liq. Cryst.286. 95-100 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Maritano, T. Kohzuma, S. Suzuki, A. Marchesini: "Stability of Ascorbate Oxidase Extracted From Cucurbita pepo" Phytochemistry. 41. 349-351 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Fujii, T. Yoshimura, H. Kamada, K. Yamaguchi, et al.: "Electron Paramagnetic Resonance Studies of Ferric Cytoclromec' from Photosynthetic Bacteria" Biochim. Biophys. Acta. 1251. 161-169 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R. Matsuzaki, S. Suzuki, K. Yamaguchi, et al.: "Spectroscopic Studies on the Mechanism of the Topa Quinone Generation in Bacterial Monoamine Oxidase" Biochemistry. 34. 4524-4530 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Yamaguchi, Deligeer, N. Nakamura et al.: "Isolation and Characterization of Two Distinct Azurins from Alcaligenes xylosoxidans subsp. xylosoxidans NCIB 11015 or GIFUI 051" Chem. Lett.353-354 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木晋一郎(日本化学会編): "NO-化学と生物(化学総説No. 30)" 学会出版センター, 126-133 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木晋一郎(桜井弘編): "バイオサイエンスESR, Vol. 2" 広川書店, 412-425 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinnichiro Suzuki, Deligeer, Kazuya Yamaguchi, Kunishige Kataoka, Kazuo Kobayashi, Seiichi Tagawa, Takamitsu Kohzuma, Sohsuke Shidara, Hidekazu Iwasaki: "Spectroscopic Characterization and Intramolecular Electron Transfer Processes of Native and Type 2 Cu-deleted Nitrite Reductases" J.Biol.Inorg.Chem.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Silvana Maritano, Augusto Marchesini, Shinnichiro Suzuki: "Spectroscopic Characterization of Native and Co (II) -substituted Zucchini Mavicyanin" J.Biol.Inorg.Chem.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsuhiko Yoshimura, Satoshi Fujii, Hitoshi Kamada, Kazuya Yamaguchi, Shinnichiro Suzuki, Sohsuke Shidara, Susumu Takakuwa: "Spectroscopic Characterization of Nitrosylheme in Nitric Oxide Complexes of Ferric and Ferrous Cytochrome c'from Photosynthetic Bacteria" Biochim.Biophys.Acta. 1292. 39-46 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoon-Ho Choi, Ryuichi Matsuzaki, Shinnichiro Suzuki, and Katsuyuki Tanizawa: "Role of Conserved Asn-Tyr-Asp-Tyr Sequence in Bacterial Copper/2,4,5-Trihydroxy-phenylalanyl Quinone-containing Histamine Oxidase" J.Biol.Chem.271. 22598-22603 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeshi Sakurai, Fumitaka Nose, Takayuki Fujiki, and Shinnichiro Suzuki: "Reduction and Oxidation Processes of Blue Copper Proteins, Azurin, Pseudoazurin, Umecyanin, Stellacyanin, Plantacyanin, and Plastocyanin Approached by Cyclic and Potential Step Voltammetries" Bull.Chem.Soc.Jpn.69. 2855-2862 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takamitsu Kohzuma and Shinnichiro Suzuki: "Spectroscopic and Electrochemical Studies on the Four-a-helices Bundle Cytochrome c'" Mol.Cryst.Liq.Cryst.286. 95-100 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Silvava Maritano, Takamitsu Kohzuma, Shinnichiro Suzuki, and Augusto Marchesini: "Stability of Ascorbate Oxidase Extracted From Cucurbita pepo" Phytochemistry. 41. 349-351 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoshi Fujii, Tetsuhiko Yoshimura, Hitoshi Kamada, Kazuya Yamaguchi, Shinnichiro Suzuki, Sohsuke Shidara, Susumu Takakuwa: "Electron Paramagnetic Resonance Studies of Ferric Cytochrome c' from Photosynthetic Bacteria" Biochim.Biophys.Acta. 1251. 161-169 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ryuichi Matsuzaki, Shinnichiro Suzuki, Kazuya Yamaguchi, Toshio Fukui, and Katsuyuki Tanizawa: "Spectroscopic Studies on the Mechanism of the Topa Quinone Generation in Bacterial Monoamine Oxidase" Biochemistry. 34. 4524-4530 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuya Yamaguchi, Deligeer, Nobuhumi Nakamura, Sohsuke Shidara, Hidekazu Iwasaki, and Shinnichiro Suzuki: "Isolation and Characterization of Two Distinct Azurins from Alcaligenes xylosoxidans subsp.xylosoxidans NCIB11015 or GIFUI051" Chem.Lett.1995. 353-354

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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