1996 Fiscal Year Final Research Report Summary
Molecular Genetic Analyzes of an Arabidopsis thaliana Mutant that Over-accumulates Soluble methionine.
| Project/Area Number |
07454214
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
NAITO Satoshi Hokkaido Univ.Fac.of Agr.Asso.Pro., 農学部, 教授 (20164105)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Masayuki Hokkaido Univ.Fac.of Agr.Pro., 農学部, 助教授 (70192482)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Arabidopsis thaliana / methionine / amino acid synthesis / ethionine resistant / systathionine gamma-synthase / mutant |
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| Research Abstract |
We have previously isolated an Arabidopsis thaliana mutant, mtol-1 that over-accumulates soluble methionine. The objectives of this research project is to disclose the mechanism of regulation of amino acid biosynthesis in higher plant by using the mtol-1 mutant. Northern analyzes indicated that the mRNA for cystathionine gamma-synthase gene was higher in the mutant than in wild-type plants. On the other hand, mRNA levels for cystathionine beta-lyase, methionine synthase (putative), and SAM syntheses are not appreciably affected by the mutation. Application of methionine from the root repressed the accumulation of cystathionine gamma-synthase mRNA in wild-type plants, while no such effect was evident in mtol-1 mutant plants. The results suggests that wild-type plants have a system to repress the level of cystathionine gamma-synthase mRNA and that the regulation system is impaired in the mtol mutant.
Both the mtol mutation and cystathionine gamma-synthase gene mapped to the top region of chromosome 3, suggesting that the mtol mutation cases a cis mutation that affects either the transcription of cystathionine gamma-synthase gene of the stability of the mRNA.
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