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1996 Fiscal Year Final Research Report Summary

Studies on chloroplast-dependent expression of some nuclear genes

Research Project

Project/Area Number 07454219
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionNagoya University

Principal Investigator

SUGIURA Masahiro  Nagoya University, Center for Gene Research, Professor, 遺伝子実験施設, 教授 (80027044)

Co-Investigator(Kenkyū-buntansha) SUGITA Mamoru  Nagoya University, Center for Gene Research, Assistant Professor, 遺伝子実験施設, 助教授 (70154474)
Project Period (FY) 1995 – 1996
Keywordschloroplast / genome / cDNA / tobacco / in vitro system / differential display
Research Abstract

Chloroplast development depends on coordinate expression of nuclear and chloroplast genomes. Based on genetic and physiological studies, some of nuclear genes are known to be expressed only when chloroplasts are functional. It has been proposed that functional chloroplasts send a signal to the nuclus. This study has forcused on the elucidation of such a possible chloroplast signal.
1. Identification of chloroplast-dependent nuclear genes
Fourteen cDNAs so far isolated are all expressed in a chloroplast-dependent manner. Furthermore, 100 additional cDNAs whose expression is chloroplast-dependent have been identified by differential display assay. These include psaD,psaF,rbcS and cab.
2. A system for searching a chloroplast signal
An in vitro transcription system has been developed from tobacco cultured BY-2 cells (containing non photosynthetic plastids). This supports a low level transcription of rbcS while the level increases with extracts from tobacco leaves (containing functional chloroplasts).

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] M. Sugiura: "A plant basal in vitro system supporting accurate transcription of both RNA polymerases II-and III-dependent genes : supplement of green leaf component (s) drives accurate transcription of a light-responsive rbcS genes" EMBOJ.14. 1024-1031 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Sugiura: "In vitro transcription of plant RNA polymerase I-dependent rRNA genes is species-specific" Plant J.8. 295-298 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugiura, M: "Plant in vitro transcription : the opening of a new era." Trends in Plant Sci.1. 41- (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fan, H. and M. Sugiura: "Basal and activated in vitro transcription in plants by RNA polymerase II and III." Methods in Enzymol.273. 268-277 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Vera. A., T. Hirose and M. Sugiura: "A ribosomal protein gene (rp132) from tobacco chloroplast DNA is transcribed from alternative promoters: similarities in promoter region organization in plastid housekeeping genes." Mol. Gen. Genet.251. 518-525 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Kapoor, J. Y. Suzuki and M. Sugiura: "Identification and functional significance of a new class of non-consensus type plastid promoters." Plant J.11. 101-111 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugita, M. and M. Sugiura: "Regulation of gene expression in chloroplasts of higher plants. In ″Post-Transcriptional Control of Plant Gene Expression″" Kluwer Academic Publishers, 315-326 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Sugiura: "Plant in vitro transcription systems. Annu. Rev. Plant Physiol. Plant Mol. Biol." Annu. Rev. Inc., 383-398 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Sugiura: "A plant basal in vitro system supporting accurate transcription of both RNA polymerases II-and III-dependent genes : supplement of green leaf component(s) drives accurate transcription of a light-responsive rbcS genes." EMBO J.14. 1024-1031 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Sugiura: "In vitro transcription of plant RNA polymerase I-dependent rRNA genes is species-specific." Plant J.8. 295-298 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugiura, M.: "Plant in vitro transcription : the opening of a new era." Trends in Plant Sci.1. 41 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fan, H.and M.Sugiura: "Basal and activated in vitro transcription in plants by RNA polymerase II and III." Methods in Enzymol.273. 268-277 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Vera, A., T.Hirose and M.Sugiura: "A ribosomal protein gene(rp132)from tobacco chloroplast DNA is transcribed from alternative promoters : similarities in promoter region organization in plastid housekeeping genes." Mol.Gen.Genet.251. 518-525 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kapoor, J.Y.Suzuki and M.Sugiura: "Identification and functional significance of a new class of non-consensus type plastid promoters." Plant J.11. 101-111 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugita, M.and M.Sugiura: "Regulation of gene expression in chloroplasts of higher plants." In"Post-Transcriptional Control of Plant Gene Expression". 315-326 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Sugiura: "Plant in vitro transcription systems." Annu.Rev.Plant Physiol.Plant Mol.Biol.383-398 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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