1996 Fiscal Year Final Research Report Summary
Studies on chloroplast-dependent expression of some nuclear genes
| Project/Area Number |
07454219
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Nagoya University |
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Principal Investigator |
SUGIURA Masahiro Nagoya University, Center for Gene Research, Professor, 遺伝子実験施設, 教授 (80027044)
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| Co-Investigator(Kenkyū-buntansha) |
SUGITA Mamoru Nagoya University, Center for Gene Research, Assistant Professor, 遺伝子実験施設, 助教授 (70154474)
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| Project Period (FY) |
1995 – 1996
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| Keywords | chloroplast / genome / cDNA / tobacco / in vitro system / differential display |
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| Research Abstract |
Chloroplast development depends on coordinate expression of nuclear and chloroplast genomes. Based on genetic and physiological studies, some of nuclear genes are known to be expressed only when chloroplasts are functional. It has been proposed that functional chloroplasts send a signal to the nuclus. This study has forcused on the elucidation of such a possible chloroplast signal.
1. Identification of chloroplast-dependent nuclear genes
Fourteen cDNAs so far isolated are all expressed in a chloroplast-dependent manner. Furthermore, 100 additional cDNAs whose expression is chloroplast-dependent have been identified by differential display assay. These include psaD,psaF,rbcS and cab.
2. A system for searching a chloroplast signal
An in vitro transcription system has been developed from tobacco cultured BY-2 cells (containing non photosynthetic plastids). This supports a low level transcription of rbcS while the level increases with extracts from tobacco leaves (containing functional chloroplasts).
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