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1997 Fiscal Year Final Research Report Summary

Molecular mechanisms at the nerve net formation in hydra primitive nervous system

Research Project

Project/Area Number 07454230
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionFukuoka Women's University

Principal Investigator

KOIZUMI Osamu  Fukuoka Women's Univ., Environmental Science, Professor, 人間環境学部, 教授 (50094777)

Co-Investigator(Kenkyū-buntansha) MINOBE Sumiko  Fukuoka Women's Univ., Environmental Science, Assistant, 人間環境学部, 助手 (80190718)
Project Period (FY) 1995 – 1997
Keywordsdiffuse nervous system / hydra / nerve net formation / developmental neurobiology / peptide / immunohistochemistry / antibody production / nerve differntiation
Research Abstract

(1) Large scale screening of peptide signal molecules : According to our peptide project, the following ideas were clarified.
(i) Peptide molecules have developmental functions such as morphogenesis and cell differentiation in addition to the well known function, the transmission of neural signals.
(ii) Peptide molecules are epithelial peptides localized in epithelial cells in addition to neuropeptides localized in nerve cells.
(2) Chemical anatomy of the hydra nervous system using neuropeptide antibodies : We produced antibodies to all peptides which structures were identified. Using these antibodies, we did chemical anatomy of hydra, and the detailed anatomical chart covering the whole body was able to be made.
(3) Molecular mechanisms of the nerve net formation : We identified peptide molucules controlling the nerve differentiation. Hym355 is a neuropeptide activating the nerve differentiation. LPW peptides are epithelial peptides inhibiting the nerve differentiation.
(4) Nerve cell culture system as a bio-assay system. We tried to establish the cell culture system of nerve cells. If it will be established, it will be used as a bioassay system to screen neuroactive substances or active substances influences on the nervous system. The present situation is a success of short term culture of single neurons.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] S.Yum: "A novel neuropeptide, Hym-176, induces contraction of the ectodermal muscle in hydra"Biochemical. Biophysical. Research. Communication. 248. 584-590 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takahashi: "Systematic isolation of peptide signal molecules regulating development in hydra I LWamide and Rlo families"Proc. Natl. Acad. Sci. USA. 94. 1241-1246 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takahashi: "Structure-activity relation of LWamide peptides synthesized with a multipeptide synthesizer"Peptide Chemistry. 1996. 193-196 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Amano: "Morphogenesis of the atrichous isorhiza, a type of nematocyst, in Hydra observed with a monoclonal antibody"Dev. Genes. Evol.. 207. 413-416 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murate: "Scanning electron microscopy of endodermal sensory cells of Hydra magnipapillata"Cell and Tissue Research. 283. 455-459 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minobe: "Nerve cell differentiation in nerve-free tissue of epithelial hydra from precursor cells introduced by grafting"Developmental Biology. 172. 170-181 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小早川義尚: "動物の血液細胞と生体防御(和合編、菜根出版)"腔腸動物の組織不適合成と細胞性防御. 232 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yum, S., Takahashi, T., Koizumi, O., Ariura, Y., Kobayakawa, Y., Mohri, S., & Fujisawa, T.: "A novel neuropeptide, Hym-176, induces contraction of the ectodermal muscle in hydra"BBRC. 248. 584-590 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano H., Koizumi O. & Kobayakawa Y.: "Morphogenesis of nematocyst, Atrichous isorhiza, in Hydra observed with a monoclonal antibody"Dev. Genes. 207. 413-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, T., Muneoka, Y., Koizumi, O. et. al.: "Systematic isolation of peptide signal molecules from hydra : LWamide and PW families"Proc. Natl. Acad. Sci. USA. 94. 1241-1246 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, T., Muneoka, Y., and Koizumi, O. et. al.: "Structure-activity relation of LWamide peptides synthesized with a multipeptide synthesizer"Peptide Chemistry 1996. 193-196 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murate,M., Takahashi-lwanaga, H., Kurosaki, R., Takeda, M.,and Koizumi, O.: "Scanning electron microscopy of endodermal sensory cells of Hydra magnipapillata"Cell Tissue Res. 283. 455-459 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi, O.: "Regeneration of hydra (in Japanese)"Genetics. 50, no. 4. 38-44 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minobe, S., Koizumi, O. and Sugiyama, T.: "Nerve cell differentiation in nerve-free tissue of epithelial hydra from precursor cells introduced by grafting. I. Tentacles and hypostome"Developmental Biology. 172. 170-181 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayakawa, Y. & Koizumi, O, ed. by K. Wago: "Histoincompatibility and cellular defense in coelenterate (in Japaneses). in "Animal Blood Cells and Host Defence""Saikon-Suppan, Tokyo, Japan. 21-40 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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