1997 Fiscal Year Final Research Report Summary
Generation of transgenic tobacco plants with enhanced drug-metabolism coupled with photosynthesis
| Project/Area Number |
07456030
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | Kobe University |
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Principal Investigator |
OHKAWA Hideo Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (90233048)
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| Co-Investigator(Kenkyū-buntansha) |
IMAISHI Hiromasa Kobe University, Faculty of Agriculture, assistant, 農学部, 助手 (50223318)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Herbicide Resistance / Chloroplast / Transgenic tobacco / P450 Monooxygenase |
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| Research Abstract |
The transgenic tobacco plants expressing rat P4501A1 and its fused enzyme with yeast P450 reductase in chloroplants were generated by genetic engineering. P4501A1 and its fused enzyme were coupled with photosynthetic electron transport chains in the chloroplants to exhibite an enhanced monooxygenase activity under light irradiation. The transgenic tobacco plants metabolized the herbicide chlorotoluron through N-demethylation and m-methyl hydroxylation to exhibite herbicide resistance. Particularly, m-methyl hydroxylation seemed to be important for herbicide resistance.
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