1996 Fiscal Year Final Research Report Summary
The unique gene structure of GART and its regulation of exprssion in the brain
| Project/Area Number |
07456043
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ONODERA Kszukiyo The University of Tokyo, Graduate School of Agriculture and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (90012773)
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| Project Period (FY) |
1995 – 1996
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| Keywords | human chromosome / gene for Purine synthesis / Down Syndrome / brain |
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| Research Abstract |
Cloning of a human cDNA encoding the trifunctinal glycinamide ribonucleotide synthetase (GARS) -aminoimidazole ribonucleotide synthetase (AIRS) -glyucinamide ribonucleotide transformylase (GART) enzyme by functional complementation in E.coli and in yeast has been reported previously. In this manuscript we describe the isolation of a novel cDNA clone encoding only the GARS domain from a human fetal brain cDNA library using the 5' region of the human GARS-AIRS-GART-cDNA as a probe. Analysis of the nucleotide sequence reveals that the coding region of this cDNA is identical to that of the GARS domain of the GARS-AIRS-GART gene cDNA.Analysis of the expression patterns of the two transcripts in normal human brains of various ages demonstrates that each is transcribed at different overall levels depending upon age. The results show that the human GARS-AIRS-GART gene produces alternatively spliced transcripts as has been shown for the Drosophia, chicken, and mouse genes. Moreover, the transcripts appear to be differentially regulated.
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