1996 Fiscal Year Final Research Report Summary
DIRECT IMAGING OF E.coil POLYMERASE AND TRANSCRIPTION COMPLEX WITH PROMOTER REGION OF MUTANT DNA (UV5).
| Project/Area Number |
07457005
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nagoya University |
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Principal Investigator |
USUKURA Jiro Associate professor of Department of anatomy Nagoya University School of Medicine, 医学部, 助教授 (30143415)
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| Co-Investigator(Kenkyū-buntansha) |
OBATA Shuichi Assistant of Department of anatomy Nagoya University School of Medicine, 医学部, 助手 (10204273)
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| Project Period (FY) |
1995 – 1996
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| Keywords | low angle shadowing / DNA / transcription / RNA polymerase / CREB / CRP / E.coil / Electron microscopy |
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| Research Abstract |
Improved low angle rotary shadowing was available for structural analysis of RNA polymerase and its binding to DNA (transcription complex). The shadowed image of the polymerase was basically composed of four subunits in two different sizes and one rod like structure. A dimeric structure of two big subunits was stacked on another small dimeric structure, forming large channel between them, 3nm in width. Rod like structure was found in this channel. Big and small subunits seem to correspond to b, b^ユ and 2 a subunits respectively, judging from differences in their molecular weight that have been revealed biochemically so far. On binding with the promoter region of DNA,DNA strand was found in this large channel. Comparative observation of core enzyme without s factor suggested that s factor should be rod like structure placed along this channel together with DNA.Another narrow space was recognizable between b and b^ユ subunits. Taking the affinity of b subunit to mRNA into account, this slit may be a channel for newly synthesizing mRNA.
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