1996 Fiscal Year Final Research Report Summary
Physiological roles of vasopressin and its receptor subtypes in the central nervous system.
| Project/Area Number |
07457025
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Fukuoka University |
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Principal Investigator |
KAMIYA Hirro-o Fukuoka Univ., Faculty of Pharmaceutical Sciences, Prof., 薬学部, 教授 (90028366)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Kenji Fukuoka Univ., Faculty of Pharmaceutical Sciences Assist Prof., 薬学部, 助手 (60140761)
SAITO Ryo Fukuoka Univ., Faculty of Pharmaceutical Sciences Assist Prof., 薬学部, 助手 (80122696)
TAKANO Yukio Fukuoka Univ., Faculty of Pharmaceutical Sciences, Asso. Prof., 薬学部, 助教授 (50113246)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Vasopressin Receptor / Vasopressin / V_1 receptor antagonist / Hippocampus / V_1 receptor mRNA / V_2 receptor mRNA |
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| Research Abstract |
We previously reported that V_2 receptor mRNA only was detected in the hippocampus of rat, although the characteristics of vasopressin (AVP) receptor based on the ^3H-AVP binding experiment using the membrane preparation of rat hippocampus were of V_1 receptor. This discrepancy indicates the possible existence of a novel AVP receptor subtype other than V_<1a>, V_<1b> or V_2 in the hippocampus. In this project, we set about to survey the characteristics of AVP receptor subtypes cprresponding to diverse roles in the brain.
(1) The subtypes of AVP receptor in some areas observed in this study were as follows : (1) The sensitivity of baroreceptor reflex in the area postrema (AP), while an increase in the sensitivity of the baroreceptor reflex in the initial stage of a one-kidney one clip (1K 1C) hypertensive rats was reversed by microinjection of V_1 or V_2 antagonists into the AP.(2) RT-PCR assay was carried out for measurement of AVP receptor mRNA from 1K 1C rat brain. V_2 receptor PCR products were detected in the initial stage of 1K 1C rats in the AP and the nucleus tractus solitarius. These results suggest that AVP receptor subtypes in the AP play very important roles in development of some kind of hypertension. (3) V_1 receptor was involved in the impairment of spatial cognition with an AVP metabolize, AVP 4-9.
(2) AVP caused an increase of ACTH release from the anterior pituitary cultured cell via an AVP receptor subtype other than V_<1a> or V_2.
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[Publications] Shimohigashi, Y., Tani, A., Matsumoto, H., Nakashima, K., Yamaguchi, Y., Oda, N., Takano, Y., Kamiya, H., Kishino, J., Arita, H.and Ohno, M.: "Lysine-49-phospholipases A_2 from Trimeresurus flavoviridis venom are membrane-acting enzymes." J.Biochem.118. 1037-1044 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Mizuta, A., Takano, Y., Honda, K., Saito, R., Matsumoto, T.and Kamiya, H.: "Nitric oxide is a mediator of tachykinin NK3 receptor-induced relaxation in rat mesenteric artery." Br. J.Pharmacol.116. 2919-2922 (1995)
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[Publications] Katsuragi, T., Matsuo, K., Sato, C., Honda, K., Kamiya, H.and Furukawa, T.: "Non-neuronal release of ATP and inositol 1,4,5-triphosphate accumulation evoked by P_2-and M-receptor stimulation in guinea pig ileal segments" J.Pharmacol. Exp. Ther.277. 747-752 (1996)
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[Publications] Eguchi, T., Takano, Y., Hatae, T., Saito, R., Nakayama, Y., Shigeyoshi, Y., Okamura, H., Krause, J.E.and Kamiya, H.: "Antidiuretic action of tachykinin NK-3 receptor in the rat paraventricular nucleus." Brain Res.743. 49-55 (1996)
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[Publications] Nakayama, Y., Takano, Y., Eguchi, K., Migita K., Saito, R., Tsujimoto, G.and Kamiya, H.: "Modulation of the arterial baroreceptor reflex by the vasopressin receptor in the area postrema of the hypertensive rats." Naurosci. Lett.(in press). (1997)
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「研究成果報告書概要(欧文)」より
