| Project/Area Number |
07457044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Sapporo Medical University School of Medicine |
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Principal Investigator |
KANOH Hideo Sapporo Medical University School of Medicine, Department of Biochemistry, Professor, 医学部, 教授 (70045475)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Shin-ichi Sapporo Medical University School of Medicine, Department of Biochemistry, Instr, 医学部, 助手 (20213209)
KAI Masahiro Sapporo Medical University School of Medicine, Department of Biochemistry, Instr, 医学部, 助手 (80260777)
SAKANE Fumio Sapporo Medical University School of Medicine, Department of Biochemistry, Assis, 医学部, 講師 (10183815)
WADA Ikuo Sapporo Medical University School of Medicine, Department of Biochemistry, Assoc, 医学部, 助教授 (40182969)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Diacylglycerol kinase / Phosphatidic acid phosphatase / signal transduction / Phospholipids / cDNA cloning |
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| Research Abstract |
We have been working on diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP). We succeeded for the first time in the cDNA cloning of mouse 35-kDa PAP,and subsequently cloned two human PAP isozymes designated PAP-2a and 2b.Our work showed that PAP is unexpectedly involved in the regulation of germ cell migration and cellular differentiation.
We also described the cDNA cloning of DGK-delta. We analyzed the translocation of DGK-alpha upon T-lymphocyte activation, and also identified the catalytic domain of this DGK isozyme. We investigated the calcium-binding properties of EF-hand motifs present in common in alpha-, beta- and gamma isoforms of DGK.
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