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1996 Fiscal Year Final Research Report Summary

Morphologic studies

Research Project

Project/Area Number 07457052
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionTokai University School of Medicine

Principal Investigator

OSAMURA Yoshiyuki  Tokai University Department of Pathology Professor, 医学部, 教授 (10100992)

Co-Investigator(Kenkyū-buntansha) TAKEKOSHI Susumu  Tokai University Department of Pathology Instructor, 医学部, 助手 (70216878)
SATO Shinkichi  Tokai University Department of Pathology Assistant Professor, 医学部, 講師 (80119677)
TSUTSUMI Yutaka  Tokai University Department of Pathology Associate Professor, 医学部, 助教授 (80138643)
Project Period (FY) 1995 – 1996
KeywordsPituitary gland / Pit-1 / Receptor / Immunohistochemistry / In situ hydridization / mRNA / In situ PCR / in situ PCR
Research Abstract

In order to clarify the mechanisms of specific functional expression of the anterior pituitary cells, we have investigated the expressoins of Pit-1 isoforms. Pit-1 beta and Pit-1 T by immunohistochemistry (IHC) and in situ hydridization (ISH). In the human pituitary and pituitary adenomas, our results suggested the involvement of Pit-1 beta in the differentiation toward GH and Pit-1 T toward TSH,respectively.
Recently, the synergetic roles of GH releasing hormone (GHRH) and receptor for estrogen (ER), for thyroid hormone (TR) and for retinoid (RAR,RXR) with Pit-1 have been reported and emphasized. By Combined ISH-IHC histochemical staining, ER and RXR were colocalized with Pit-1 in the same cells. In prolactin secreting adenomas, Pit-1 and ER were colocalized in the same cells. Regarding GHRH receptor (GHRHR), we are in the process of introducing in situ RT-PRCR in order to detect trivial amount of GHRHR mRNA.Preliminary, GHRHR has been co-localized in the same cells of the hyperplastic pituitary gland in hGHRH transgenic rats.
Our studies have suggested the synergetic effects of Pit-1 isoforms and various receptors in the specific functional expressions of the anterior pituitary cell and in the pituitary adenoma cells. More detailed mechanisms remain to be investigated in the future investigations.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Matsuno A.,Osamura RY,et al.: "HGH,PRL and ACTH gene expression in clinically non-functioning adenomas detected with non-isotopic in situ hybridization" Humanol Pathol. 6. 13-20 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuno A.,Osamura RY,et al.: "Changes in the ultrastructural distribution of prolactin and growth hormone mRNAs in pituitary cells of f.rats after estrogen and bromocriptine treatment." Acta Neuropathol Histochem Cell Biol. 104. 37-45 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamura RY.,Sanno N.et al.: "Clinical and cytofunctional classification of pituitary adenomas:Proposal of a new classification" Endocrine Pathology. 6. 253-256 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sanno N.,Osamura RY.et al.: "Studies on GH and PRL gene expression by Non-radioisotopic in situ hybridization in TSH-secreting pituitary adenomas." J Clin Endocrinol Metab. 80. 2518-2522 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sanno N.,Osamura R.Y.et al.: "Expression of Pit-1 and Estrogen Receptor Messenger Ribonucleic Acids in Prolactin Producing Pituitary Adenomas." Modern Pathology. 9. 529-533 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sanno N.,Osamura R.Y.et al.: "Application of CSA in immunodetection of gonadotropin subunits in clinically functioning pituitary adenomas" Am J Clin Pathol. 106. 16-21 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sanno N., Osamura RY., Matsuno A., Teramoto A: "Immunocytochemical studies of pituitary adenomas in clinical cytology." J Jpn Soc Clin Cytol. 34. 111-115 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuno A., Osamura RY,et.al: "HGH,PRL and ACTH gene expression in clinically non-functioning adenomas detected with non-isotopic in situ hybridization." Endocr Pathol. 6. 13-20 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuno A., Osamura RY,et.al: "Expression of plurihormonal mRNAs in somatotroph adenomas detected using a non-isotopic in situ hybridization method" Hum Pathol. 26. 272-279 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuno A., Osamura RY,et.al: "Changes in the ultrastructural distribution of prolactin and growth hormone mRNAs in pituitary cells of female rats after estrogen and bromocriptine treatment." Histochem Cell Biol. 104. 37-45 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Genka S,Osamura RY,et.al: "Acromegaly, diabetes insipidus and visual loss caused by metastatic Growth Hormone-Releasing-Hormone (GRH) producig undifferentiated malignant pancreatic endocrine tumor in the pituitary gland." J.Neurosurg. 83. 719-723 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sanno N,Osamura RY., et.al: "Studies on GH and PRL gene expression by Non-radioisotopic in situ hybridization in TSH-secreting pituitary adenomas." J Clin Endocrinol Metab. 80. 2518-2522 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abe T,Matsumoto K,Sanno N,Osamura Y: "Lymphocytic Hypophysitis : Case report." Neurosurgery. 36. 1016-1019 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osamura RY., Sanno N., Teramoto A: "Clinical and cytofunctional classification of pituitary adenomas : Proposal of a new classification." Endocrine Pathology. 6. 253-256 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sanno N., Osamura R.Y., et.al: "Expression of Pit-1 product in human pituitary adenomas. Histochemical studies by antibody against synthetic human Pit-1" Arch Pathol Lab Med. 120. 73-77 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sanno N., Osamura R.Y., et.al: "Combined Non-isotopic in situ hybridization method and indirect immunohistochemical analysis of hormone production in rat pituitary." J Clin Pathol (Mol Pathol). 49. M57-M60 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sanno N., Osamura R.Y., et.al: "In situ Hybridizaytion Analysis of Pit-1 mRNA and Hormonal Production in Human Pituitary Adenomas." Acta Neuropathol. 91. 263-268 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sanno N., Osamura R.Y., et.al: "Expression of Pit-1 and Estrogen Receptor Messenger Ribonucleic Acids in Prolactin Producing Pituitary Adenomas." Modern Pathology. 9. 529-533 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sanno N., Osamura R.Y., et.al: "Application of CSA in immunodetection of gonadotropin subunits in clinically non-functioning pituitary adenomas." Am J Clin Pathol. 106. 16-21 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sanno N., Teramoto A., Osamura R.Y: "Clinical and cytofunctional classification of pituitary adenomas." Acta Neurochirurgica (Wien). 138. 1186-1192 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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