1996 Fiscal Year Final Research Report Summary
The role of reduced expression of glutathione peroxidase gene on the development and progression of hepatocellular carcinoma
| Project/Area Number |
07457065
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Hokkaido University |
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Principal Investigator |
MORIUCHI Tetsuya Cancer Inst.Div.Cell Biol., Hokkaido University Professor, 医学部, 教授 (20174394)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Futoshi Cancer Inst.Div.Cell Biol., Hokkaido University Instructor, 医学部, 助手 (00250423)
HAMADA Junichi Cancer Inst.Div.Cell Biol., Hokkaido University Assist.Prof, 医学部, 講師 (50192703)
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| Project Period (FY) |
1995 – 1996
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| Keywords | LEC rat / p53 / glutathione peroxidase / hepatitis |
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| Research Abstract |
The Long-Evans Cinnamon (LEC) rat is a mutant strain in which hepatitis and liver cancer spontaneously develop at extremely high rates. We observed marked reduction in the activity of cellular glutathione peroxidase (GSH-Px) in the liver of LEC rats and p53 knockout mice. To determine whether GSH-Px promoter is activated by p53, liciferase assay was performed. Activation of GSH-Px promoter was found in both cell llines expressing wild-type p53 and mutant p53, indicating that GSH-Px promoter is activated by N-terminal activation domain of p53. To investigate the role of selenium-dependent GSH-Px in the development of hereditary hepatitis, we compared incidences and grades of acute hepatitis between the LEC rats fed with seleniumm-supplemented diet and selenium-deficient diet. We found that selenium-deficient status and resultant GSH-Px deficiency does not promote hepatitis but rather have an inhibitory effect on the development of hepatitis in LEC rats.
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[Publications] Nakayama, N., Kashiwazaki, H., Kobayashi, N., Hamada, J., Matsumoto, K., Nakamura, T.and Takeichi, N: "Differing distribution of hepatocyte growth factor-positive cells in the liver of LEC rats with acute hepatitis and hepatoma." Jpn.J.Cancer Res. 86. 5-9 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Kobayashi, M., Hamada, J., Li, Y-Q., Shinobu, N., Imamura, M., Okada, F., Takeichi , N.and Hosokawa, M.: "A possible role of 92 kDa type IV collagenase in the extramedullary tumor formation in leukemia." Jpn.J.Cancer Res. 86. 298-303 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Hamada, J., Nagayasu, H., Takayama, M., Kawano, T., Hosokawa, M.and Takeichi, N: "Enhanced effect of epidermal growth factor on pulmonary metastasis and in vitro invasion of rat mammary carcinoma cells." Cancer Lett.89. 161-167 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Namieno, T,Konaka, S., Okada, F., Hamada, J., Hosokawa, M., Uchino, J.and Sato, N: "Pathological comparison between spontaneously developed and chemically induced liver cancers in LEC rats with hereditary hepatitis" Int.J.Oncol. 8. 791-794 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Shibata, T., Kawano, T., Nagayasu, H., Okumura, K., Arisue, M., Hamada, J., Takeichi, N.and Hosokawa, M: "Enhancing effects of epidermal growth factor on human squamous cell carcinoma motility and matrix degradation but not growth" Tumor Biol. 17. 168-175 (1996)
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[Publications] Kuramitsu, Y., Nishibe, M., Kobayashi, M., Togashi, Y., Yuan, L., Takizawa, M., Okada, F and Hosokawa, M: "Transforming growth factor beta 1 (TGF-beta1) produced in tumor tissue after chemotherapy acts as a lymphokine-activated killer attractant" Br.J.Cancer. 74. 274-279 (1996)
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[Publications] Yoshimura, S., Suemizu, H., Nomoto, Y., Sakai, H., Katsuoka, Y., Kawamura, M.and Moriuchi, T: "Plasma glutathione peroxidase deficiency caused by renal dysfunction" Nephron. 73. 207-211 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakayama, N., Kashiwazaki, H., Kobayashi, N., Hamada, J., Ogiso, Y., Itakura, Y., Matsumoto, K., Nakamura, T., Koike, T., Kuzumaki, N.and Takeichi, N.: "Hepatocyte growth factor and c-met expression in Long-Evans Cinnamon rats with spontaneous hepatitis and hepatoma" Hepatology. 24 (3). 596-602 (1996)
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[Publications] Ohnishi, Y., Fujii, H., Kimura, F., Mishima, T., Murata, J., Tazawa, K., Fujimaki, M., Okada, F., Hosokawa, M.and Saiki, I.: "Inhibitory effect of a traditional Chinese medicine, Juzen-taiho-to, on progressive growth of weakly malignant clone cells derived from murine fibrosarcoma." Jpn.J.Cancer Res. 87. 1039-1044 (1996)
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[Publications] Taniguchi, Y., Katsumata, Y., Koido, S., Suemizu, H., Yoshimura, S., Moriuchi, T., Okumura, K., Kagotani, K., Taguchi, H., Imanishi, T., Gojobori, T., Inoko, H: "Cloning, sequencing, and chromosomal localization of two tandemly arranged human pseudogenes for the proliferating cell nuclear antigen (PCNA)" Mamm.Genome. 7. 906-908 (1996)
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[Publications] Wang, J., Kobayashi, M., Sakurada, K., Imamura, M., Moriuchi, T.and Hosokawa, M: "Possible roles of an adult T cell leukemia-derived factor (ADF) /thioredoxin in drug resistance of adult T cell leukemia (ATL) to Adriamycin" Blood. (in press). (1997)
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