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1997 Fiscal Year Final Research Report Summary

Molecular epidemiological analysis of the antigen diversity of Plasmodium vivax and P.falciparum

Research Project

Project/Area Number 07457069
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionOsaka Institute of Technology

Principal Investigator

TANABE Kazuyuki  Osaka Institute of Technology, Engineering, Professor, 工学部, 教授 (40047410)

Co-Investigator(Kenkyū-buntansha) KIMURA Masatsugu  Osaka City Univ.Medical School Lab.of Biophysics.Res Assoc., 医学部, 助手 (60195378)
Project Period (FY) 1995 – 1997
KeywordsMalaria / Plasmodium / Gene / Antigen diversity / MSP1 / PCR / TRAP / Gene polymorphism
Research Abstract

The diversity of surface antigens of Plasmodium vivax and P.falciparum was studied at the genetic on level field isolates from endemic areas of Thailand and Vietnam. Analysis of more than 300 isolates by PCR and DNA sequencing revealed the followings.
1.Polymorphism of the merozoite surface antigen (MSP1) gene of P.vivax : 15 Thai isolates possessed 35 permutations in the regions from ICB5 to ICB6, most of which were dimorphic substitutions. Two types of recombination bwtween type 1 and type 2 sequences were found in a polymorphic region. These results suggest that allelic diversity may be generated by recombination as found for the P.falciparum MSP1 gene.
2.Polymorphism of the merozoite surface antigen (MSP1) gene of P.falciparum : A PCR system, which identifies up to 24 different MSP1 gene types per isolate, was developed by using primers specific for allelic type sequences. In total, 17 to 21 different MSP1 gene types were identified in Vietnam and Thailand, respectively. Frequency distribution of MSP1 gene type differed significantly between the two areas. The F st analysis indicated significant divergence in variable blocks of MSP1 except block 2 between Thai and Vietnam isolates. Linkage analysis between variable blocks localized regions in the gene for frequent recombination (block 4) and less frequent recombination (blocks 3 and 5) as well as no recombination (blocks 6-16). Furthermore, Thai isolates showed 2 new polymorphic residues in the C terminus, 19 kD region of MSP1.
3.Sequencing of the thrombospondin-related anonymous protein (TRAP) gene of P.falciparum of 20 Thai isolates showed variations specific for Thai isolates and also suggested recombination in the gene.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 田辺 和裄: "マラリアワクチン開発の現状と課題" 日本臨床. 54. 2252-2260 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] O.Kaneko, K.Tanabe et al.: "Plasmodium falciparum:variation in block 4 of the precursor to the major merozoite surface protein 1 in natural populations" Experimental Parasitology. 84. 92-95 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Putaporntip K.Tanade et al.: "Interallelie recombination in the merozoite surface protein-1(MPS-1)gene of Plasmodium vivax" Molecular and Biochemical Parasitology. 84. 49-56 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Ohta, K.Tanade et al.: "Epitope analysis of human T-cell response to MSP-1 of Plasmodium falciparum in malaria-nonexposed individuals" International Archives of Allergy and lmmunology. 114. 15-22 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Fu, N.Ohta, K.Tanade et al.: "MSP1-reactive T cells in individuals in malaria endemic Solomon area and in non-immune Japanese" Parasitology International. 46. 7-16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] O.Kaneko, K.Tanade et al.: "Plasmodium falciparum:allelic variation in the merozoite surface protein 1 gene in wild isolates from southern Vietnam" Experimental Parasitology. 85. 45-57 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田辺 和裄: "マラリアワクチン-最近のトライアル結果-" Modern Physician. 17. 848-850 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Jongwutiwes K.Tanade et al.: "Variation in the thrombospondin-related anonymous protein (TRAP)gene of Plasmodium falciparum from Thai isolates" Molecular and Biochemical Parasitology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Ferreira, K.Tanade et al.: "Allelic diversity at the merozoite surface protein-1 locus of Plasmodium falciparum in clinical isolates from the southwestern Brazilian Amazon" Ameican Journal of Tropical Medicine and Hygiene. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Ferreira, K.Tanabe et al.: "Stable pattern of allellic diversity at the merozcite surface protein 1-locus of Plasmodium falciparum in clinical isolates from southern Vietnam" Jounal of Eukaryotic biolology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] O.Kaneko, et al.: "Plasmodium falciparum : variation in block 4 of the precursor to the major merozoite surface protein 1 in natural populations" Experimental Parasitology. 84. 92-95 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.Putaporntip et al.: "Interallelic recombination in the merozoite surface protein-1 (MSP-1) gene of Plasmodium vivax" Mol.Biochem.Parasitol. 84. 49-56 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Ohta, et al.: "Epitope analysis of human T-cell response to MSP-1 of Plasmodium falciparum in malaria-nonexposed individuals" Int.Arch.Allergy Immunol.114. 15-22 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Fu, et al.: "MSP1-reactive T cell in individuals in malaria endemic Solomon area and in non-immune Japanese" Parasitol.International. 46. 7-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] O.Kaneko, et al.: "Plasmodium falciparum : allelic variation in the merozoite surfaceprotein 1 gene in wild isolates from southern Vietnam" Exp.parasitol.85. 45-57 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.U.Ferreira, et al.: "Stable pattern of allellic diversity at the merozoite surface protein 1-locus of plasmodium falciparum in clinical isolates from southern Vietnam" J.Eukaryotic Biol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Jongwutiwes, et al.: "Variation in the thrombospondin-related anonymous protein (TRAP) gene of Plasmodium falciparum from Thai isolates" Mol.Biochem.Parasitol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.U.Ferreira, et al.: "Allelic diversity at the merozoite surface protein-1 locus of Plasmodium falciparum in clinical isolates from the southwestern Brazilian Amazon" Am.J.Trop.Med.Hyg.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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