1996 Fiscal Year Final Research Report Summary
The development of the treatment of autoimmune diseases by cytokine gene transfer
| Project/Area Number |
07457122
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | TOKYO MEDICAL & DENTAL UNIVERSITY |
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Principal Investigator |
MIYASAKA Nobuyuki Tokyo Medical & Dental University, Department of Medicine, Professor, 医学部, 教授 (30157622)
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| Co-Investigator(Kenkyū-buntansha) |
KOHSAKA Hitoshi Tokyo Medical & Dental Uiversity, Medical Research Institute, Associate Professo, 難治疾患研究所, 助手 (00251554)
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| Project Period (FY) |
1995 – 1996
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| Keywords | cytokine / autoimmune disease / gene therapy |
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| Research Abstract |
(1) Establishment of myelin basic protein (MBP)-specific T cell lines ;
Lymph node cells were obtained from Lewis rats immunized with MBP peptide (68-88) and cultured with a repetitive stimulation with MBP and interleukin 2. T cell lines thus obtained were transfered to naive syngeneic rats and the induction of encephalomyelitis (experimental allergic encephalomyelitis ; EAE) was confirmed.
(2) Transfer of cytokine genes to T cell lines ;
Expression vectors for cytokine genes (IL-4, IL-10, TGFbeta) were prepared and transfected into COS cells. The culture supernatants were confirmed to have corresponding cytokine activities and those from TGFbeta-transfected COS cells strongly inhibited the proliferation of MBP-stimulated T cell lines in vitro. These cell lines were transferred into Lewis rats immunized with MBP peptide to examine whether cytokine-gene transfer successfully inhibit the induction of EAE.
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