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1996 Fiscal Year Final Research Report Summary

CROSSTALKS AMONG SIGNAL TRANSDUCTION SYSTEMS IN APOPTOSIS IN RELATION WITH DEVELOPMENT DIFFERENTIATION AND AGING

Research Project

Project/Area Number 07457125
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionUniversity of the Air

Principal Investigator

KITO Shozo  University of the Air, Liberal Arts, Professor, 教養学部, 教授 (00010140)

Co-Investigator(Kenkyū-buntansha) SEMBA Jun'ichi  Univ.of the Air, Liberal Arts, Associate Professor, 教養学部, 助教授 (30183429)
Project Period (FY) 1995 – 1996
Keywordsestrogen / kainic acid / AP-1-DNA binding activity / CA^<2+> / insulin-like growth factor / Immunohistochemistry / dot blotting / in situ hybridization
Research Abstract

Estrogen is deeply associated not only with development and differentiational of neuronal cells, but also with rescue mechanisms of injured or aging neurons. Our foregoing studies revealed that estrogen caused increase of the survival rate of rat hippocampal culture neurons at concentrations of 10^<-6>-10^<-9>, while depressed the survival at higher concetrations. Basing on these results, mechanisms of neuroprotective effects of estrogen were studied on kainic acid-injured brains.
Immunohistochemical studies with use of monoclonal antibody against estrogen receptor showed marked increase of estrogen receptor-like immunoreactivities not only in the limbic system, but also in the wide areas of the cerebral cortesses. Such results were also confirmed through in situ hybridization experiments for estrogen receptor mRNA.Estrogen injection prior to a systemic administration of kainic acid-induced expression of neurotrophic factor and their mRNAs such as HGF,IGF I and II,as observed by dot blotting analysis, immunohistochemistry as well as in situ hybridization.
Through a separate experiment with single injection of estrogen, we confirmed that estrogen increased AP-1-DNA binding activity with time course up to 120 min. when observed by gel shift assay method.
These results suggested that estrogen playd roles in neuronal repair with successive proceses of increased estrogen receptors, elevation of AP-1-DNA binding activity, induction of IGF I mRNA.Neuronally differentiated PC12 cells by NGF have estrogen receptors. We noticed estrogen lenpthened the survival of PC12 cells when added together with NGF.

  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 新郷 明子: "ゲルシフトアッセイからみたエストロゲンとニコチンのクロストーク" 医学と生物学. 134/1. 3-7 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Semba,J.: "Nicotine protects against the dexamrthasone potentiation of kainic acid induced neurotoxicity in cultured hippocampal neurons." Brain Research. 735. 335-338 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Semba,J.: "Differential expression of c-fos mRNA in rat prefrontal cortex,striatum,N.accumbens and lateral septum after typical and atypical antipsychotics:an in situ hybridization study." Neurochemistry International. 29/4. 435-442 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新郷明子: "カイニン酸傷害脳に対するestrogenの影響" 神経化学. 35/6. 510-511 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新郷明子: "エストロゲンと脳可塑性に関する研究" 医学と生物学. 133/5. 189-193 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新郷明子: "カイニン酸とAP-1 DNA結合活性-その時間的推移-" 医学と生物学. 133/5. 195-199 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Semba,J.: "Characterization of extraceiiular amino acids in striatum of freely moving rats by in vivo microdiaiysis." Journal of Neural Transmission. 100. 39-52 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Semba,J.: "Different expression of c-fos mRNA in rat striatum,N.accumbens and prefrontal cortex after haloperidol,suipiride and clozapine:an in situ hybridization study." Japanese Journal of Pharmacology. 67. 143 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kito,S.: "Effects of estrogen on cultured limbic neurons." Japanese Journal of Pharmacology. 67. 157 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Semba,J.: "N^G-monomethyl-L-arginine,alnhibitor of nitric oxide synthase,increases extracellular GABA in the striatum of the freely moving rat." Neuro Report. 6. 1426-1428 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Semba,J.: "Behavioural and neurochemical effect of OPC-14597,a novel antipsychotic drug,on dopaminergic mechanisms in rat brain." Neuropharmacology. 34. 785-791 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Semba,J.: "Effect of systemic dexamethasone on extracellular amino acids(glutamate,aspartate,glycine and GABA)and acetylcholine release in rat hippocampus in vivo microdialysis." Functional Neurology. 10. 17-21 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shingo, A.S.: "Estrogen-nicotine cross-talk in rat brain" Medicine and Biology. 134. 3-7 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Semba, J.: "Nicotine protects against the dexamrthasone potentiation of kainic acid-induced neurotoxicity in cultured hippocampal neurons." Brain Research. 735. 336-338 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Semba, J.: "Differential expression of c-fos mRNA in rat prefrontal cortex, striatum, N.accumbens and lateral septum after typical and atypical antipsychotics : an in situ hybridization study." Neurochemistry International. 29/4. 435-442 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shingo, A.S.: "Effect of estrogen on the kainic acid-injured brain" J.J.of Neurochemistry. 35. 510-511 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shingo, A.S.: "Estrogen plays a role in neuronal plasticity at injured brains" Medicine and Biology. 133/5. 189-193 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shingo, A.S.: "Studies on kainic acid induced AP-1 DNA binding activities-the time course-" Medicine and Biology. 133/5. 195-199 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Semba, J.: "Characterization of extracellular amino acids in striatum of freely moving rats by in vivo microdialysis." Journal of Neural Transmission. 100. 39-52 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Semba, J.: "Differential expression of c-fos mRNA in rat striatum, N.accumbens and prefrontal cortex after haloperidol, sulpiride and clozapine : an in situ hybridization study." Japanese Journal of Pharmacology. 67. 143 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kito, S.: "Effects of estrogen on cultured limbic neurons." Japanese Journal of Pharmacology. 67. 157 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Semba, J.: "N^G-monomethyl-_L-arginine, ainhibitor of nitric oxide synthase, increases extracellular GABA in the striatum of the freely moving rat." Neuro Report. 6. 1426-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Semba, J.: "Behavioral and neurochemical effect of OPC-14597, a novel antipsychotic drug, on dopaminergic mechanisms in rat brain." Neuropharmacology. 34. 785-91 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Semba, J.: "Effect of systemic dexamethasone on extracellular amino acids (glutamate, aspartate, glycine and GABA) and acetylcholine release in rat hippocampus in vivo microdialysis." Functional Neurology. 10. 17-21 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Semba, J.: "Characterization of extracellular amino acids in striatum of freely moving rat using in vivo microdialysis." Japanese Journal of Pharmacology. 64. 229 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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