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1996 Fiscal Year Final Research Report Summary

Studies on Epithelial-mesenchymal Interaction in Gastrointestinal Tract.

Research Project

Project/Area Number 07457134
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionOsaka University

Principal Investigator

KAWANO Sunao  Department of Medicine, Osaka University, Associate Professor., 医学部, 助教授 (60133138)

Co-Investigator(Kenkyū-buntansha) TAKEI Yoshiyuki  University Hospital., Osaka University, Medical Staff, 医学部・附属病院, 医員
TSUJI Shingo  University Hospital., Osaka University, Medical Staff, 医学部・附属病院, 医員
NAGANO Kouichi  Department of Medicine, Osaka University, Assistant Professor., 医学部, 助手 (60237542)
Project Period (FY) 1995 – 1996
Keywordsgastric mucosa / proliferation / differentiation / 3-dimensional culture / epithelial-mesenchymal / interaction / growth factor
Research Abstract

In this study, we investigated epithelial-mesenchymal interaction between gastrointestinal (GI) epithelial cells and fibroblasts using a three dimensional culture system. All the fibroblast cell line examined (NIH 3T3, Sweiss 3T3, MRC-5, NRK) produced an activity that promote growth/survival of non-transformed epithelial cells from the Gl tract (RGM-1, IEC-6, and primary fetal rabbit gastic epithelial cells). Non-trasformed epithelial cell lines and primary gastric epithelial cells secreted an activity that promote growth/survival and morphological transformation of the fibroblasts, whereas this activity was lost in transformed epithelial cell lines (MKN-28,45, DLD-1, HepG2, COS-1).
RGM-1, a non-transformed gastric epithelial cell line, produced an activity (s) that promote growth/survival and morphological transformation of fibroblasts and Ito cell and stimulates neurite outgrowth of PC-12 neuronal cell. To purify this activity (s), we collected 30 L of serum-free conditioned medium from large scale culture of RGM-1 cell. The activity was purified by a combination of heparin affinity, ion exchange, gel filtration, and reverse phase chromatography using a FPLC system. The purified activity has a molecular weight of 30 kDa and its pertial amino acid sequence was novel.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Sasayama K. et al.: "Kelationship between interleukin-8 and myeloperoxidase activity in human gastric mucosa." J Gastroenterol Hepatol. 12. 104-108 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tuji S, et al: "Helicobactor pylori extract stimulates inflammatory nitric oxide production" Cancer Letters. 108. 195-200 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawano S, et al: "Gastric mucosal energy metabolism and intracellular mucin content changes in patients with liver cirrhosis." J Gastroenterol Hepatol. 11. 380-384 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi I, et al: "RGM-1, a new cell line derived from normal gastric mucosa of rat." In Vitro Cell Dev Biol-Animal. 32. 259-261 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishimura Y, et al: "The F(ab)^2 fragment of an anti-ICAM-1 monoclonal antibody attenuates liver injury after orthotopic liver transplantation." Transplantation. 61. 99-104 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masuda E, et al: "Endogenons nitric oxide modulates ethanol-induced gastric mucosal injury in rats." Gastroenterology. 108. 58-64 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasayama, Kawano S,et al: "Relationship between interleukin-8 and myeloperoxidase activity in human gastric mucosa" J Gastroenterol Hepatol. 12. 104-108 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuji S,et al: "Helicobacter pylori extract stimulates inflamatory nitric oxide production" Cancer Letters. 108. 195-200 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S,et al: "Gastric mucosal energy metabolism and intracellular mucin content changes in patients with liver cirrhosis" J Gastroenterol Hepatol. 11. 380-384 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi I,et al: "RGM-1, a cell line derived from normal gastric mucosa of rat" In Vitro Cell Dev Biol-Animal. 32. 259-261 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishimura Y,et al: "The F (ab) 2 fragment of an anti-ICAM-I monoclonal antibody attenuates liver injury after orthotopic liver trans -plantation" Transplantation. 61. 99-104 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuji S,et al: "Evidences of involvement of cyclooxygenase-2 in proliferation of two gastric cancer cell lines" Prostaglandins, Leukotrienes and Essential Fatty Acids. 55. 179-183 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masuda E,et al: "Endogeneous nitric oxide modulates ethanol-induced gastric mucosal injury in rats" Gastroenterology. 108. 58-64 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsujii M,et al: "Mechanism for ammonia-induced promotion of gastric carcinogenesis in rats" Carcinogenesis. 16. 563-566 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S,et al: "Effect of loxoprofen, a newly synthesized non-steroidal anti-inflamatory drug, and indomethacin on gastric mucosal hemodynamics in he human" J Gastroenterol Hepatol. 10. 81-85 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato N,et al: "Gastric blood flow in ulcer diseases" Scand J Gastroenterol. 30 (suppl 208). 14-20 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuji S,et al: "Gastric mucosal cell proliferation and TGF-alpha in the healing of peptic ulcers in human subjects" J Clin Gastroenterol. 21 (suppl 1). S78-S81 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuji S,et al: "Role of hepatocyte growth factor and its receptor in gastric mucosa : a cell biological and molecular biological study" Dig Dis Sci. 40. 1132-1139 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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