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1996 Fiscal Year Final Research Report Summary

Cytokine receptor expression and gene in myelodysplastic syndromes.

Research Project

Project/Area Number 07457230
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

OHNO Ryuzo  Hamamatsu University School of Medicine, Professor, 医学部, 教授 (70093002)

Co-Investigator(Kenkyū-buntansha) YANAGI Mitsuaki  Hamamatsu University School of Medicine, Senior Resident, 医学部附属病院, 医員
TOBITA Tadasu  Hamamatsu University School of Medicine, Senior Resident, 医学部附属病院, 医員
TAKESHITA Akihiro  Hamamatsu University School of Medicine, Research Associate, 医学部附属病院, 助手 (00242769)
OHNISHI Kazunori  Hamamatsu University School of Medicine, Assistant Professor, 医学部附属病院, 講師 (80252170)
Project Period (FY) 1995 – 1996
KeywordsMDS / erythropoietin receptor / thrombopoietin receptor / CD34 / CD38 / glycophorin A / glycoprotein IIb / IIIa / IIIa
Research Abstract

In hematological diseases, abnormal expression of cytokine receptors and disorder of postreceptor signal transduction have been reported. However, little is known regarding the expression levels of cytokine receptors on cells in clinical samples because of the low level of expression and the difficulty in isolation of objective cells. In this study, we analyzed expression levels of erythropoietin (EPO) receptors and thrombopoietin (TPO) receptors on various phenotypic cells using a newly-devised non-isotopic ligand assay and evaluated its disorder in myelodysplastic syndromes (MDS). Some cell lines or bone marrow cells were incubated with biotinylated EPO or TPO followed by staining with streptavidine-RED670 conjugates and the fluorescence intensity was measured by a glow cytometer. The expression level of EPO receptors on cells was evaluated with the fluorescence intensity and the D balue calculated by the Kolmogorov-Smirnov statistics. By the simultaneous staining with anti-CD34, CD38, glycophorin A or glycoprotein IIb/IIIa, the expression levels of EPO receptors and TPO receptors on each phonotypic cells were analyzed. The specificity of this assay was confirmed by an experiment of cell lines EPO receptors were detected quantitatively in CD34 and/or glycophorin A positive fractions and TPO receptors were detected quantitatively in CD34 and/or glycoprotein IIb/IIIa positive fractions. The expression levels of both EPO receptors and TPO receptors on cells from MDS patients were decreased. In conclusion, the expression of EPO receptors on erythroid cells and TPO receptors on megakaryocytes were able to be detected quantitatively by non-isotopic ligand assay. This assay was specific and sensitive, and was performed easoly. The expression levels of both EPO receptors and TPO receptors on cells from MDS patients were decreased, and thus gene therapy introducting these receptor genes into MDS cells may be warranted.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Kanamaru, A., Ohno, R. et al.: "All-trans retinoic acid for the treatment of newly diagnosed acute promyelocytic leukemia." Blood. 85. 1202-1206 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohnishi, K., Ohno, R. et al.: "A randomized trial comparing interferon-α with busulfan for newly diagnosedchronic myelogenous leukemia in chronic phase." Blood. 86. 906-916 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinjo, K., Ohno, R. et al.: "Expression of granulocyte colony-stimulating factor receptor increases with differentiation in myeloid cells by a newly-devised puantitative flow-cytometric assay." Br. J. Haematol.91. 783- (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi, T., Ohno, R. et al.: "Randomized trials between behenoyl cytarabine and bytarabine in combination induction and consolidation therapy, and with or without ubenimex after maintenance/intensification therapy in adult acute myeloid luekemia." J. Clin. Oncol.14. 204-213 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubo, K., Ohno, R. et al.: "Different T-cell receptor repertories between lesions and peripheral blood in acute graft-versus-host disease after allogeneic bone marrow transplantation." Blood. 87. 3019-3026 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshita, A., Ohno, R. et al.: "Successful treatment of relapse of acute promyelocytic leukemia with a new synthetic retinoid, Am80." Ann. Intern. Med.124. 893-896 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinjo, K., Ohno, R. et al.: "Erythropoietin receptor expression on human bone marrow erythroid precursor cells by a newly-devised quantitative flow-cytometric assay." Br. J. Haematol.96. 551-558 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinjo, K., Ohno, R. et al.: "Ganulocyte colony-stimulating factor receptro at various differentiation stages of normal and leukemic hematopoietic cells." Leukemia and Lymphoma. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanamaru, A., Ohno, R.et al.: "All-trans retinoic acid for the treatment of newly diagnosed acute promyelocytic leukemia." Blood. 85. 1202-1206 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohnishi, K., Ohno, R.et al.: "A randomized trial comparing interferon-alpha with busulfan for newly diagnosedchronic myelogenous leukemia in chronic phase." Blood. 86. 906-916 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinjo, K., Ohno, R.et al.: "Expression of granulocyte colony-stimulating factor receptor increases with differentiation in myeloid cells by a newly-devised puantitative flow-cytometric assay." Br. J.Haematol.91. 783-794 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi, T., Ohno, R.et al.: "Randomized trials between behenoyl cytarabine and bytarabine in combination induction and consolidation therapy, and with or without ubenimex after maintenance/intensification therapy in adult acute myeloid luekemia." J Clin. Oncol.14. 204-213 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kubo, K., Ohno, R.et al.: "Different T-cell receptor repertories between lesions and peripheral blood in acute graft-versus-host disease after allogeneic bone marrow transplantation." Blood. 87. 3019-3026 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeshita, A., Ohno, R.et al.: "Successful treatment of relapse of acute promyelocytic leukemia with a new synthetic retinoid" Am80. Ann. Intern. Med.124. 893-896 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinjo, K., Ohno, R.et al.: "Erythropoietin receptor expression on human bone marrow erythroid precursor cells by newly-devised quantitative flow-cytometric assay." Br. J.Haematol.96. 551-558 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinjo, K., Ohno, R.et al.: "Ganulocyte colony-stimulating factor receptro at various differentiation stages of normal and leukemic hematopoietic cells." Leukemia and Lymphoma. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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