1996 Fiscal Year Final Research Report Summary
Constrution of cDNA bank from bispsy speeinens for unltiple gene analyis of cancer
| Project/Area Number |
07457259
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | YOKOHAMA CITY UNIVERSITY |
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Principal Investigator |
SIMADA Hirosi Yokohama City Univ. , School of Medicine, Professor, 医学部・第2外科, 教授 (90117747)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIKAWA Yasusi Yokohama City Univ. , School of Medicine, Assistant, 医学部・第2外科, 助手 (70254208)
YAMAOKA Hiroyuki Yokohama City Univ. , School of Medicine, Lecture, 医学部・第2外科, 講師 (90230317)
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| Project Period (FY) |
1995 – 1996
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| Keywords | cDNA Bank / mRNA / Cancer |
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| Research Abstract |
The "Cancer cDNA Bank" of colorectal cancer patients was constructed with our unique mRNA assay system.
Total nucleic acids extracted from colorectal cancers were applied to 96-well plates, where poly-d (T) sequences of oligonucleotides were immobilized. After hybridization, the ds-cDNA was synthesized on the plate using the captured mRNA as a template. The resultant sense cDNA removed from the plate was used for RT-PCR for analysis of various genes. The sense strand of the cDNA was repeatedly synthesized using the immobilized antisense cDNA as a template even after storage.
Furthermore, the results of RT-PCR can be easily compared among different specimens, if the same amount of total mRNA was applied to the plate for the ds-cDNA synthesis.
These demonstrated that the "Cancer cDNA Bank" constructed from clinical materials provides almost unlimited supplies of cDNA for multiple gene analysis.
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