1996 Fiscal Year Final Research Report Summary

Attempts to immortalize vascular smooth muscle cells for the elucidation of the pathogenesis of moyamoya disease

Research Project

Project/Area Number	07457314
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Cerebral neurosurgery
Research Institution	KYOTO UNIVERSITY
Principal Investigator	KIKUCHI Haruhiko Kyoto University, School of Medicine, Professor, 医学研究科, 教授 (20072746)
Co-Investigator(Kenkyū-buntansha)	HOSHIMARU Minoru Kyoto University, School of Medicine, Assistant professor, 医学研究科, 講師 (70211539) NAGATA Izumi Kyoto University, Schoool of Medicine, Assistant professor, 医学研究科, 講師 (10198327)
Project Period (FY)	1995 – 1996
Keywords	Moyamoya Disease / Superficial Temporal Artery / Immortalization / v-myc / TGF-beta1 / HoxB7
Research Abstract	Although the primary lesion of moyamoya disease is stenosis of the intracranial arterial trunk, the superficial temporal artery (STA) has been suggested to be involved in moyamoya disease. Immortalization of vascular smooth muscle cells derived from the STA,which was obtained at surgery, was attempted using LINXv-myc retrovirus in order to obtain enough material for molecular biological studies. Although smooth muscle cells infected with the LINXv-myc retrovirus has not been immortalized, they were able to be cultured several times longer than original cells.