• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

Effect of volatile anesthetics on the ion-channels of the cerebral vascular smooth muscle isolated from dog

Research Project

Project/Area Number 07457361
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionWakayama Medical College

Principal Investigator

HATANO Yoshio  Wakayama Medical College, Department of Anesthesia, Professor, 医学部, 教授 (70115913)

Co-Investigator(Kenkyū-buntansha) MIZUMOTO Kazuhiro  Wakayama Medical College, Department of Anesthesia, Assistant Professor, 医学部, 助手 (50239258)
KAKUTANI Tetsuya  Wakayama Medical College, Department of Anesthesia, Assistant Professor, 医学部, 助手 (00264896)
Project Period (FY) 1995 – 1996
KeywordsCerebral artery / Mesenteric artery / Ca^<++>-activated K^+ channel / Na^+-K^+ ATPase / Voltile anesthetics
Research Abstract

(1) The rings of the dog cerebral artery without endothelium but not the mesenteric artery maintained an automatic myogenic tone in the resting state. The myogenic tone was abolished in the Ca^<++> free Ringer solution and in the presence of nifedipine. These findings suggest that the increased basal Ca^<++> influx through voltage-dependent Ca^<++> channel is responsible for the maintenance of myogenic tone in the cerebral artery. Halothane significantly inhibited the myogenic tone observed in the cerebral arterial rings.
(2) The addition of tetraethylammonium and charybdotoxin (Ca^<++>-activated K^+ channel inhibitors) produced a concentration-dependent contraction in the cerebral artery, but not in the mesenteric artery. These findings suggest that Ca^<++>-activated K^+ channel was highly activated by the increased Ca^<++> influx in the cerebral artery. Halothane, even at a low concentration of 0.5 MAC,strongly attenuated the tension development elicited by tetraethylammonium and charybdotoxin.
(3) Exposure of the mesenteric arterial rings precontracted with phenylephrine to halothane (4 MAC) caused a rapid contraction, which reached a plateau within 5 to 10 min. After stabilization, termination of halothane produced a transient and profound relaxation. This relaxation was inhibited by ouabain, a Na^+-K^+ ATPase inhibitor, in a concentration-dependent manner. Substitution of Li^+ for Na^+ in Ringer solution also attenuated the relaxation. These results suggest that the relaxation elicited by termination of halothane exposure may be responsible to an alteration of Na^+-K^+ ATPaes activity in the mesenteric artery.
The effect of volatile anesthetics on the alteration of intracellular ion concentration (Na^+, Ca^<++>) is now under way in our laboratory.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Yoshio Hatano et al: "Enclothelium-dependent and-independent vasodilation of isolated rat aorta indoced by caffeine" Am.J.Physiol.269. H1679-H1684 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura K.et al: "Vasodilative effect of adrenomedollin in isolated arteries of the dog" Jpn J.Pharmacol. 67. 259-262 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iranami H,Hatano Y,et al.: "A beta-adrenoreceptor agonist evokes a nitric oxide -CGMP relaxation mechanism modvlated by adenylate cyclase in rat aorta" Anesthesiology. 85. 1129-1138 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ooka T,Hatano Y et al.: "Protective effects of human urinary trypsin inhibitor in rat aorta" Crt Care Med. 24. 1903-1907 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogawa K et al: "Volatile anaesthetics ottencate hypocopnia-induced confriction in isolared dog cerebral orteries" Can J.Anaesth. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto M,Hatano Y. et al: "Different effects of halothan,Isoflurome and sevaflurane on sarcoplasmic reticulum of vosculor smooth muscle in dog mexenteric" Acta Anaesth Scand. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshio, Hatano, Kazuhiro Mizumoto, Takeshi Yoshiyama, Manabu Yamamoto, Hiroshi Iranami: "Endothelium-dependent and -independent vasodilation of isolated rat aorta induced by caffeine" Am J Physiol. 269. H1679-1684 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura K,Toda H,Terasako K,Kakuyama M,Hatano Y,Mori K,Kanagawa K: "Vasodilative effect of adrenomedullin in isolated arteries of the dog" Jpn J Pharmacol. 67. 259-262 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirakata H,Ushibiki F,Narumiya S,Hatano Y,Nakamura K,Mori K: "The effects of inhaled anesthetics on the platelet aggregation and ligand-binding affinity of the platelet thromboxane A2 receptor" Anesth Analg. 81. 114-119 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iranami H,Hatano Y,Tsukiyama Y,Maeda H,Mizumoto K: "A beta-adrenoreceptor agonist evokes a nitric oxide-cGMP relaxatoin mechanism modulated by adenylate cyclase in rat aorta" Anesthesiology. 85. 1129-1138 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ooka T,Hatano Y,Yamamoto M,Ogawa K,Saika S: "Protective effects of human urinary trypsin inhibitor in rat aorta" Crit Care Med. 24. 1903-1907 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirakata H,Ushikubi F,Toda H,Nakamura K,Sai S,Urabe N,Hatano Y,Narumiya S,Mori K: "Sevoflurane inhibits human platelet aggregation and thromboxane A2 formation, possibly by supression of cyclooxygenase activity" Anesthesiology. 85. 1447-1453 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa K,Yamamoto M,Mizumoto K,Hatano Y: "Volatile anaesthetics attenuate hypocapnia-induced constriction in isolated dog cerebral arteries" Can J Anaesth. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto M,Hatano Y,Kakuyama M,Nakamura K,Tachibana T,Maeda H,Mori K: "Different effects of halothane, isoflurane and sevoflurane on sarcoplasmic reticulum of vascular smooth muscle in dog mesenteric artery" Acta Anaesth Scand. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iranami H,Hatano Y,Tsukiyama Y,Yamamoto M,Maeda H,Mizumoto K: "Inhibitory action by halothane on acethylcholine-induced relaxation is less potent in mesenteric artery" Can J Physiol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi