1997 Fiscal Year Final Research Report Summary
Effects of rG-CSF on Proliferation of Urological Cancer Cells
| Project/Area Number |
07457374
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
NAITO Katsusuke Yamaguchi University School of Medicine, Professor, 医学部, 教授 (60115251)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIHIRO Satoru Yamaguchi University School of Medicine, Assistant, 医学部, 助手 (40284260)
YAMAMOTO Mitsutaka Yamaguchi University School of Medicine Hospital, Assistant Professor, 医学部・附属病院, 講師 (80243640)
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| Project Period (FY) |
1995 – 1997
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| Keywords | rG-CSF / Urological malignant tumor cells / Proliferation / in vitro |
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| Research Abstract |
The cultivation of PBMCs with OK-432 inhibited the production of rG-CSF by PBMCs. The inhibition may play a role in the mechanism of the cytokine-mediated antitumor effect of OK-432.
Tumor cells such as KK-47 cells and T24 cells, and PBMCs were seeded in separated agar layr each other in the following experiments. Any cytokines such as IL-6, EGF,basic-FGF,IL-2 were not detected in the supernatant of cultured PBMCs. KK-47 cells constitutively produced basic-FGF and IL-6 in the supernatant and T24 cells produced IL-6. When KK-47 cells were cultured with rG-CSF for 96 hours, productio of basic-FGF by KK-47 cells increased in a dose-dependent manner until rG-CSF concentraion of 10 ng/ml. Furthermore, the production of basic-FGF increased under the presence of both of PBMCs and rG-CSF in the culture medium. When basic-FGF was added into culture medium, cell number estimated by MTT assay of KK-47 cells increased in a dose-dependent manner on basic-FGF concentrations. Cell number of KK-47 cells cultured with both of rG-CSF and PBMCs significantly increased than those cultured with rG-CSF only. Cell number of S-phase fraction estimated by FCM of KK-47 cells increased in cultivation with rG-CSF,however, that of T24 cells did not in the same condition. In RT-PCR,KK-47 cells expressed mRNAs of IL-6 receptor, FGF receptor 1 and rG-CSF receptor, however, no expression of mRNA of FGF receptor 2 was observed. T24 cells expressed mRNA of IL-6, however, no expression of mRNA of rG-CSF was observed. PBMCs expressed mRNAs of rG-CSF,FGF receptor 1 and 2. It was suggested that IL-6 and jbasic FGF might be autocrine factors of KK-47 cells. Production of basic FGF by KK-47 cells activated by rG-CSF might take part in effect of rG-CSF on proliferation of KK-47 cells.
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