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1996 Fiscal Year Final Research Report Summary

Expression of basic FGF,FGF receptor, TGF-beta, and VEGF in experimental choroidal neovascularization

Research Project

Project/Area Number 07457418
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKansai Medical University

Principal Investigator

UYAMA Masanobu  Kansai Medical University, School of Medicine, Professor, 医学部, 教授 (30025580)

Co-Investigator(Kenkyū-buntansha) MIYASHIRO Miki  Kansai Medical University, School of Medicine, Instructor, 医学部, 助手 (00288838)
YAMADA Haruhiko  Kansai Medical University, School of Medicine, Instructor, 医学部, 助手 (50288841)
OGATA Nahoko  Kansai Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (60204062)
TAKAHASHI Kanji  Kansai Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (60216710)
Project Period (FY) 1995 – 1996
Keywordsangiogenesis / choroidal neovascularization / Vascular endothelial growth factor (VEGF) / Fibroblast growth factor (FGF) / FGF-receptor / Transforming growth factor-beta (TGF-beta) / in situ hybridization / retinal pigment epithelium
Research Abstract

Development and regression of choroidal neovascularizaion (ChNV) may be regulated by growth factors and cytokines. To reveal existence and localization of basic fibroblast growth factor (b FGF), FGF receptor, transforming growth factor (TGF), vascular endothelial growth factor (VEGF) in ChNV,we demonstrated expression of mRNAs of them with in situ hybridization method and immunohistochemical study.
ChNV were produced in pigmented rat with intense photocoagulation (PC). Antisense and sense riboprobes were prepared from rat b FGF,FGF receptor, TGF-beta_1, mouse TGF-beta_2, TGF-beta_3, VEGF cDNAs. In situ hybridization was performed on sections of the retina and choroid following PC.Basic FGF,FGF receptor, TGF-beta_1, TGF-beta_2, and VEGF,mRNAs were detected in the ganglion cell layr in normal rat retina, though TGF-beta_3 was not detected. In developing stage of ChNV after PC,expression of b FGF,FGF receptor and VEGF mRNAs were marked in the retinal pigment epithelial cells, and vascular endothelial cells. Expression of TGF-beta_1 and TGF-beta_2, mRNAs were also detected. However, these factors were no longer detected in regressing stage of ChNV.
These results suggest endogenous b FGF,and VEGF promotes development of choroidal neovascularization, and TGF-beta_1 and beta_2 appears to be associated in regulation of ChNV,in vivo.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Ogata N, et al.: "Expression of transforming growth factor-β mRNA in experimental choroidal neovascularization" Current Eye Research. 16. 9-18 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogata N, et al.: "Expression of basic fibroblast growth factor mRNA in developing choroidal neovascularization" Current Eye Research. 15. 1008-1018 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yi X, et al.: "Immunohistochemical proof of origin of macrophages in laser phtocoagulation in the retina" Japanese Journal of Ophthalmology. 40. 192-201 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsushima M, et al.: "FGF receptor 1 expression in experimental choroidal neovascularization" Japanese Journal of Ophthalmology. 40. 329-338 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto C, et al.: "Immunolocalization of basic fibroblast growth factor during wound repair in rat retina after laser photocoagulation." Graefe's Arch Clin Exp Ophthalmology. 234. 695-702 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto C, et al.: "Gene Expressions of basic fibroblast growth factor and its receptor in healing of rat retina after laser photocoagulation" Japanese Journal of Ophthalmology. 40. 480-490 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yi X, et al.: "Vascular endothelial growth factor expression in choroidal neovascularization in rats." Graefe's Arch Clin Exp Ophthalmology. 235. 313-319 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本 千加子 他: "網膜光凝固後の創傷修復過程における塩基性線維芽細胞増殖因子とその受容体の発現-In situ hybridization法における検討-" 日本眼科学会雑誌. 100(4). 270-278 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松島 正史 他: "実験的脈絡膜新生血管形成過程における線維芽細胞増殖因子レセプター1発現のin situ hybridizationによる証明" 日本眼科学会雑誌. 99(6). 642-648 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸部 隆雄 他: "インターフェロンβの実験的脈絡膜新生血管に対する効果" 日本眼科学会雑誌. 99(5). 571-581 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸部 隆雄 他: "網膜光凝固後の網膜色素上皮修復過程へのインターフェロンβの効果" 日本眼科学会雑誌. 99(7). 792-805 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸部 隆雄 他: "ラットにおける実験的脈絡膜新生血管の長期経過" 日本眼科学会雑誌. 99(7). 784-791 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸部 隆雄 他: "網膜光凝固後の脈絡膜毛細血管板再形成過程へのインターフェロンβの効果" 日本眼科学会雑誌. 99(5). 558-570 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogata N,et al: "Expression of transforming growth factor-beta mRNA in experimental choroidal neovascularization" Current Eye Research. 16. 9-18 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogata N,et al: "Expression of basic fibroblast growth factor mRNA in developing choroidal neovascularization" Current Eye Research. 15. 1008-1018 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yi X,et al: "Immunolocalization proof of origin of macrophages in laser photocoagulation in the retina" Japanese Journal of Ophthalmology. 40. 192-201 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsushima M,et al: "FGF receptor I expression in experimental choroidal neovasculazation" Japanese Journal of Ophthalmology. 40. 329-338 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto C,et al: "Immtinohistochemical of basic fibroblast growth factor during wound repair in rat retina after laser phtocoagulation" Graefe's Arch Clin Exp Ophthalmology. 234. 695-702 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto C,et al: "Gene Expression of basic fibroblast growth factor and its receptor in healing of rat retina after laser phtocoagulation" Japanese Journal of Ophthalmology. 40. 480-490 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yi X,et al: "Vascular endothelial growth factor expression in choroidal neovascularization in rats" Graefe's Arch Clin Exp Ophthalmology. 235. 313-319 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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