1996 Fiscal Year Final Research Report Summary
Ditribution of bone matrix proteins and PTH recepter mRNA during ectopic bone formation induced by BMP
| Project/Area Number |
07457428
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
NAGATSUKA Hitoshi Okayama University Dental School Associate Professor, 歯学部, 助教授 (70237535)
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| Co-Investigator(Kenkyū-buntansha) |
AKAGI Takumi Okayama University Dental School Research Assistant, 歯学部, 教務員 (50192878)
MURATA Masaru Okayama University Dental School Assistant, 歯学部, 助手 (00260662)
INOUE Masahisa Okayama University Dental School Assistant, 歯学部, 助手 (20223274)
NAGAI Noriyuki Okayama University Dental School Professor, 歯学部, 教授 (90085770)
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| Project Period (FY) |
1995 – 1996
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| Keywords | BMP / mRNA / in situ hybridization / bone matrix protein / collagen / non RI / PTH prcepter |
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| Research Abstract |
Purtialy purified bovine BMP-collagen I : 1 W after implantation. Osteopontin mRNA expression was found mainly at the central area of the tissue. At higher magnification, strong osteopontin mRNA expression was seen in large ovary shaped cells Osteonectin mRNA expression was found in the central to the middle area of the tissue. Osteonectin mRNA expression seen in fibroblast-like cells. Osteocalcin mRNA expression could not be detected at 1W after implantatin. Type I collagen mRNA expresison was found in all area. 2 W after implantation. Strong osteopontin and osteonectin mRNA expression was found in almost all areas of the tissue. At higher magnificaiton, strong osteonectin mRNA expression was seen in chondrocytes, osteoblasts, and young osteocytes. Osteocalcin mRNA expression was found in osteoblasts and osteoxytes of the newly formed bone. Type II and X collagen mRNA expression was found in chondrocytes and few osteoblast. 3 W after implantation. Osteopontin mRNA expression was found
… More
in osteoblasts surrounding the trabecular-shaped woven bone. Strong osteonectin mRNA expression was found in osteoblasts an osteocytes. Osteocalcin mRNA expression was found in osteoblasts and young osteocytes. Type I collagen mRNA espression was found in osteoblasts and young osteocytes.
rhBMP-2-IMB : In the heterotopic cartilage like tissue, Osteopontin, osteocalcin mRNA and Type II Collagen were detected in chondrocyte-like cells. Whileosteocalcin mRNA was absent in chondrocytes in the enodchondral ossification of the fetal rat tibia. These results suggest that the chondrocyte-like cells in the BMP induced cartilage like tissue demonstrate the two phase funciton of both chondrocytes and osteoblast. The inductive cartilage-like tissue may be deisgnated as chondro-osseous cells in stead of chondro-cytes. The heterotopic chondro-osseous tissue formation induced by rhBMP-2 is process that differs from that of normal endchondral ossification in respect to cell differentiation and function. Less
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