1996 Fiscal Year Final Research Report Summary
Role of BMP in fracture repair
| Project/Area Number |
07457432
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
YAMAGUCHI Akira School of Dentistry, Showa University, Associate Professor, 歯学部, 助教授 (00142430)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKI Shusaku School of Dentistry, Showa University, Professor, 歯学部, 教授 (30085740)
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| Project Period (FY) |
1995 – 1996
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| Keywords | regeneration / fracture repair / BMP / Hedgehog / osteoblusts |
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| Research Abstract |
1) We investigated the histological changes occurred during fracture repair of ribs in 6-week old mice. On day 3 after fracture, chondrocytes appeared at fracture sites. ALP-positive osteoblasts first appeared at the fracture sites on day 4 after fracture.
2) The expression of mRNAs of BMP-2, BMP-4 and Sonic hedgehog (Shh) were up-regulated on days 1 and 2 after fracture.
3) Shh induced osteoblastic differentiation in C3H10T1/2 and MC3T3-E1 cells.
4) The transplantation of the fibroblasts expressing Shh into muscular sites in nude mice induced ectopic bone formation.
These results demonstrated that BMPs and Shh play important roles during fracture repair.
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