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1997 Fiscal Year Final Research Report Summary

Function of cell adhesion molecules and cytokine in the periodontal disease.

Research Project

Project/Area Number 07457453
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

WATANABE Hisashi  Tokyo Med Dent Univ., Dentistry, Assoc Prof., 歯学部, 助教授 (40143606)

Co-Investigator(Kenkyū-buntansha) NAGASAWA Toshiyuki  Tokyo Med Dent Univ., Dentistry, Res.assis., 歯学部, 助手 (90262203)
NOGUCHI Kazuyuki  Tokyo Med Dent Univ., Dentistry, Res.assis., 歯学部, 助手 (90218298)
Project Period (FY) 1995 – 1997
Keywordsclonal anergy / Th2 cytokine / COX-2 / human periodontal ligament cell / ALP gnen
Research Abstract

T cell clones across an IFN-gamma endothelial cell (EC) barrier in vitro. Transmigration was greatly enhanced after stimulation by Aa and antigen presenting cells (APC). Other stimuli (Con A,IL-2) did not enhance transmigration. Blocking of a second signal provided by APC (with CTLA4-Ig) significantly reduced transmigration. Also, blocking of the adhesion melecule LFA-1 on Th1 or Th2 clones with monoclonal antibody resulted in 75-97% obstruction of transmigration. IFN-gamma treatment of EC resulted in dramatic upregulation of class II MHC expression suggesting that EC could present antigen. IFN-gamma significantly increased Th1 clone transmigration. IL-4 decreased Th1 trasmigration, but enhanced Th2 transmigration. Anergy of transmigrated antigen-specific T lymphocytes can be a protective mechanism that limits extensive Th1 cell proliferation to bacterial antigens and the resultant production of potentially destructive IFN-gamma and IL-2. Transmigration anergy may also play a role in peripheral Tcell tolerance by limiting proliferation of autoreactive gingival T cells. The purpose of the second study was to investigate the involvement of cyclooxygease-1 (COX-1) and cyclooxygenase-2 (COX-2) in prostaglandin (PG) production by human periodontal ligament (PDL) fibroblasts stimulated with a proinflammatory cytokine, interleukin-1beta (IL-1beta), and to examine the effect of interleukin-4 (IL-4), a Th2 cytokine, and interferon-g (IFN-gamma)、a Th1 cytokine, on PG production by the cells. This study suggests that COX-2 are responsible for PEG_2 production by IL-1beta-stimulated human PDL cells and that IL-4 anmd IFN-gamma down-regulate PEG_2 production through inhibition of COX-2 expression and with no effect on COX-2 expression. We also have obtained several results as to ALP gene.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Ishikawa I, Watanabe H et al.: "Induction of the immunu response to periodontopathic bacteria and its role in the patho pnusis of periodonfitis." Periodontology 2000. 14. 79-111 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe H.et al.: "Wound healing after irradiation of bone tissue by Er:YAG laser" SPIE. 2973. 39-42 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Goseku-Some M, Watanabe M et al.: "Hypophosphatasia:Identification of five novel miscense mutation in the tissue-Non opecific alkalime phospystose gene amoung,Japanese patients." Human Mutation. Suppl.1. S263-S267 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa I,Watanabe H et al.: "Induction of the immune response to periodontopathic bacteria and its role in the pathogenesis of periodontitis." Periodontology 2000. 14. 79-111 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe H et al.: "Wound herling after irradiation of bone tissue by Er : YAG laser." SPIC. 2973. 39-42 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goseki Sone M.Watanabe H et al.: "Hypophosphatasea : Identification of five novel missense mutation in the tissue-non specific alkaline phosphatase gene among Japanese" Human Mutation. Suppl.1. S263-S267 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16   Modified: 2017-10-10  

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