1996 Fiscal Year Final Research Report Summary
Molecular Cloning of Dentin Morphogenetic Protein
| Project/Area Number |
07457460
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | KYUSHU UNIVERSTIY |
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Principal Investigator |
NAKASHIMA Misako Kyushu Univ., Dentistry, Research Associate, 歯学部, 助手 (20207773)
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Yasuharu Kyushu Univ., Dentistry, Research Associate, 歯学部, 助手 (00170473)
AKAMINE Akifumi Kyushu Univ., Dentistry, Professor, 歯学部, 教授 (00117053)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Bone morphogenetic protein (BMP) / TGF-beta superfamily / pulpal wound healing / dentinogenesis / RT-PCR / in situ hydridization / molecular cloning |
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| Research Abstract |
BMP inductive signals may be implicated in the tooth development and repair, and our previous data has shown fifteen different members of the transforming growth factor-beta superfamily, TGF-beta1, TGF-beta2, TGF-beta3, inhibin/activinbetaA,inhibin/activin betaB,BMP-2, BMP-4, BMP-6, BMP-7, BMP-8, GDF-1, GDF-5, GDF-6, GDF-7 and GDNF are expressed in rat incisor pulp. We have further identified new members of the transforming growth factor-beta superfamily from rat incisor pulp and designated dentin morphogenetic protein (DMP)-62 and 63. A degenerate primer set derived from the highly conserved carboxy-terminal region of TGF-beta superfamily was used for RT-PCR with poly (A)^+ RNA from the rat incisor pulp as template. The mature carboxyl-terminal domain of DMP-62 and DMP-63 are 44% identical to rat vgr-1 and 46% identical to human inhibin/activin betaB respectively. DMP-63 also shows significant homology to inhibin/activin betaB (approximately 25% amino acid sequence identity) in the pro-region of the molecules. Northern blot analyzes showed that DMP-63 is predominantly expressed in dental pulp and brain. In situ hybridization of sections from rat incisor pulp tissue showed that DMP-63 was predomonantly expressed at the stage of preodontoblasts and odontoblasts just before and after mineralization, suggesting a potential role for DMP-63 in tooth development and repair.
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