• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

Studies on the role of epidermal growth factor receptors in the proliferation of tumorigenic submandibular gland epithelial cells in vitro and in vivo

Research Project

Project/Area Number 07457488
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOsaka University

Principal Investigator

SAKUDA Masayoshi  Osaka University, Faculty of Dentistry, Professor, 歯学部, 教授 (00028755)

Co-Investigator(Kenkyū-buntansha) CHISOKU Hirohisa  Osaka University, Dental School Hospital, Resident, 歯学部・附属病院, 医員
KATO Itsuro  Osaka University, Dental School Hospital, Resident, 歯学部・附属病院, 医員
OMAE Masatoshi  Osaka University, Dental School Hospital, Resident, 歯学部・附属病院, 医員
SUMI Tetsuro  Osaka University, Faculty of Dentistry, Assistant Professor, 歯学部, 助手 (40252697)
NAKAZAWA Mitsuhiro  Osaka University, Dental School Hospital, Assistant Professor, 歯学部・附属病院, 助手 (70217701)
Project Period (FY) 1995 – 1996
KeywordsEGF receotor / Receptor overexpression / Salivary gland tumor / Tumor growth control
Research Abstract

We transfected human epidermal growth factor receptor gene into tumorigenic mouse submandibular glamd epitelial cells, and examined their changes in a proliferative capacity after the transfection in comparison with untransfected TMSG cells. Transfected cells (TMSG-EGFR) showed overexpressed hEGFR molecule on their surface. TMSG-EGFR cell proliferation was much faster than that of TMSG cekks and etimulated by EGF and TGF-alpha in dose dependent manner. TMSG-EGFR cells demonstrated a resistance to TGF-beta which strongly inhibited TMSG cell proliferation. TMSG-EGFR cells formed markedly faster and larger tumors in synergic mice than did TMSG cells. Since TMSG-EGFR cells in culture were highly resposive to EGF.Effects of EGFR on TMSG-EGFR tumor growth in vivo was examined. To study this, submandibular glands, which are known to produce more than 95% of EGF in male mice were removed. Plasma EGF levels in sialoadenectomized mice were markedly decreased for 3 to 5 weeks after surgery. TMSG-EGFR tomors shwed prfoundly retarded growth in the sialoadenectomized mice, whereas TMSG tumor growth showed no change. These results suggest that TMSG-EGFR tumor growth was influenced by circulating EGF which was secreted form submandibular glands, TMSG-EGFR tumor growth in culture and in mice was significantly diminished by a tyrosine kinase inhibitor, whereas TMSG growth was not affected by this agent. In xonxlusion, (1) overexpression of EGFR in cancer cells not only increase a responsiveness to EGF but might cause an acquisition of a responsiveness to other growth factors and a resistance to growth inhibitory factors. These changes, at least in part, might account for an increased overall proliferative capacity of cancer cells with aberrant EGFR expression. (2) there might be a possibility that pharmacologic inhibitor of tyrosine kinases are potent anti-cancer agents.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Tomohiko Ogawa: "Immuno pharmacological Activities of the non-toxic Monophosphoryl Lipid A of Porphyromonas Gingivalis" Vaccine. 14・1. 70-76 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Nishida: "Effects of Protein Phosphatase Inhibition by Okadaic Acid on the Differentiation of F9 Embryonal Cells" Exp. Cell. Res.217. 288-293 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中澤光博: "口腔扁平上皮癌の再発時期に関する検討" 頭頸部腫瘍. 21. 110-114 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉川文弘: "変形性腺腫およびその悪性型(悪性変形性腺腫、変形性腺腫内痕)の臨床的特徴の比較検討" 日本口腔外科学会雑誌. 42. 590-592 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TOMOHIKO OGAWA: "Immuno pharmacological activities of the non-toxic monophosphoryl lipid A of Porphylomonas Gingivalis" Vaccine. 14-1. 70-76 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Nishina: "Effects of protein phosphatase in hibition by Okadaic acid on the differentiation of F9 embryonal cells" Experimantal Cell.Res.217. 288-293 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhiro Nakazawa: "Studied on the recurrence period of oral squamous cell carcinomas" Head and Neck Tumors. 21. 110-114 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F.Yoshikawa: "Studies on the clinical features of pleomorphic adenomas and malignant plemorphic adenoma" Jpn J.Oral Maxillo.Surg.42. 590-592 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi