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1996 Fiscal Year Final Research Report Summary

Osteonectin Degrading Activity in Calf Bone Matrix

Research Project

Project/Area Number 07457498
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionTsurumi University

Principal Investigator

ISHIBASHI Katsunori  School of Dental Medicine, Tsurumi University Professor, 歯学部, 教授 (20013980)

Co-Investigator(Kenkyū-buntansha) YAMACHIKA Shigeo  School of Dental Medicine, Tsurumi University Assistant, 歯学部, 助手 (60182565)
Project Period (FY) 1995 – 1996
Keywordscalf bone matrix / non-collagenous protein / osteonectin / proteinase
Research Abstract

Soluble proteins were sequentially extracted from demineralized calf bone matrix, with Tris buffer, NaCl solution and guanidine HCl solution. These extracts were concentrated for enzymography, but the guanidine HCl extract was muddied by this manipulation. Then the extract was separated GS (supemate) and GR (residue)-extract by centrifuge. Proteolytic activities in these extracts were investigated by means of enzymograply using gelatin or casein as a substrate. Identical proteolytic active bands were detected on the enzymograms of each the NaCl extract (N-extract), GS-extract and GR-Extract. However, when the purified osteonectin was used as substrate, a proteolytic activity was detected only in the GR-extract. These results would indicate that the presence of a specific osteonectin-lytic proteinase in bone. The enzyme needed Ca ions for activity and was inhibited by EDTA and 1,10-phenanthoroline, it would be an active type metalloproteinase which was bound to bone collagenous matrix Osteonectin degraded fragments were observed 26k, 22k, 20k, 16k, and 14k dalton bands by SDS-PAGE.Sinoe some of these bands were doserved in GR-extract, degraded fragments were bound more tighty than intact osteonectin. This result suggested that osteonectin might be degraded for increase ability of binding to collagen.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 山近重生: "ウシ骨基質中のオステオネクチン分解酵素活性に関する研究" 鶴見歯学. 22. 235-251 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeo YAMACHIKA: "Osteonectin Degrading Activity in Calf Bone Matrix" Tsurumi Univ.Dent.J.22 (2). 235-251 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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