1997 Fiscal Year Final Research Report Summary
Roles and Expression Mechanisms of Cellular Adhesion Molecules during the Initiation of Arteriosclerosis
| Project/Area Number |
07457562
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
OKADA Masahiko Niigata University, School of Medicine, Professor, 医学部, 教授 (30018915)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
INANO Koichi Niigata University Hospital, Assistant, 医学部・附属病院, 助手 (10262445)
MIIDA Takashi Niigata University Hospital, Lecturer, 医学部・附属病院, 講師 (80260545)
MATSUTO Takayuki Niigata University, School of Medicine, Asso Professor, 医学部, 助教授 (80209577)
|
|---|
| Project Period (FY) |
1995 – 1997
|
| Keywords | preventive medicine / arteriosclerosis / vascular endothelial cell / macrophage / cellular adhesion molecule / cell signaling / oxidized LDL / cytokine |
|---|
| Research Abstract |
Expressions of the adhesion molecules in arterial endothelial cells are crucial events during the initiation of arteriosclerosis. Our study showed that endothelial cells expressed endothelial leukocyte adhesion molecule-1 (ELAM-1) occasionally but significantly by oxidized LDL,glycated LDL,H_2O_2, and hypoxic culture mediumin vitro. Also significant factor was immune-complex of oxidized LDL and its auto-antibody. Next we examined temporal relations of induction of ELMA-1, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion mplecule-1 (VCAM-1) in cultured endothelial cells of human thoracic aorta. Activators examined were IL-1alpha (10ng/mL), TNFalpha (10ng/mL), and INFgamma (10ng/mL). Cells were incubated with each of the cytokines for 0.5-48 hours. ELAM-1 was observed by the activations with IL-1 and TNFalpha ; IL-1 gave a sharp rise after an hour incubation and showed the maximum expression at 2 hours, while TNFalpha showed a slow rise after 30 minutes and the maximum at 4 hours. ICAM-1 expression was observed evn in non-stimulated cells and further increased in proportion to duration of the activation. There was no significant difference between the effects of IL-1 and TNFalpha to the ICAM-1 expression. Slight expression of VCAM-1 was observed only by TNFalpha after 2-hours incubation. INFgamma did not cause any change in the expression of ELAM-1, VCAM-1, and ICAM-1. The present data indicate that there appear to be specific signal pathways for each induction of ELAM-1 and VCAM-1, but not ICAM-1. In atherogenesis, therefore, the temporal relations of the molecules may play a role for endothelial cells to discriminate monocytes from other cells such as neutrophils.
|
