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1996 Fiscal Year Final Research Report Summary

Study on the role of Lp (a) as an acute phase reactant and on the effects on arterial endothelial and smooth muscle cells.

Research Project

Project/Area Number 07457563
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionGifu University

Principal Investigator

NOMA Akio  Gifu University School of Medicine, Professor, 医学部, 教授 (30208384)

Co-Investigator(Kenkyū-buntansha) SAITO Kuniaki  Gifu University School of Medicine, Assistant, 医学部, 助手 (80262765)
SHIMOKAWA Kuniyasu  Gifu University School of Medicine, Assistant Professor, 医学部, 助教授 (70021376)
Project Period (FY) 1995 – 1996
KeywordsLipoprotein (a), Lp (a) / Acute phase reactant / Immunohistochemistry / Tissue repair / Endothelial cell / Migration / Proliferation / Angiogenesis
Research Abstract

Although we have reported transient increases of plasma lipoprotein (a) [Lp (a)] after acute myocardial infarction and surgical operations, the significance of these increases remains unclear. The purpose of the present study was to clarify the physiological roles of Lp (a) in tissue repair.
Many samples from inflamed tissues were examined by immunohistochemical techniques. The immunohistochemical features of granulation tissues were characterized by different stages of wound healing. In the first stage, immunoreactivities for anti-apo (a) and-apo B were weak and focal, whereas those for anti-plasminogen and-fibrinogen were strong and widespead on the tissue surface. In the second stage, granulation tissue were covered with loose fibrous connective tissue, and markedly positive staining for Lp (a) was observed closer to the surface, suggesting that Lp (a) may prevent external fibrinolysis. Lp (a) was also found in endothelial cells and the extracellular space of small vessels underlying the fibrous connective. tissues. In the last stage of healing, apo (a) and apo B were not detectable in completely organized tissues. These findings suggest that Lp (a) plays a role in the wound healing.
In in vitro study with human umbilical vein endothelial cell (HUVEC), the stimulatory effects of Lp (a) on the migration and proliferation of endothelial cells were observed. These effects were not affected with its isoforms. Anti-bFGF,added in the incubation medium treated with Lp (a), suppressed the Lp (a)-induced HUVEC migrations and proliferations, but the inhibitions were not complete. Pertussis toxin, that blocks the activity of bFGF,also suppressed Lp (a)-induced HUVEC migration. These findings suggest that Lp (a) stimulates the bFGF-mediated HUVEC migration and proliferation, and may promote the angiogenesis.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] K.Makino et al: "Rhesus monkey lipoprotein (a). Relationship between apo (a) isoforms and Lp (a) density in plasma." Ann. N. Y. Acad. Sci.748. 603-605 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Abe et al: "Polymorphism and distribution of apo (a)." J. Atheroscler. Thromb.2. S8-S12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 荒川博徳 ほか: "閉塞性動脈硬化症における動脈組織Lp (a)値の検討" 脈管学. 15 (6). 552-555 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野間昭夫: "生体の急性相反応蛋白の分子生物学: Lipoprotein (a), Lp (a)" 動脈硬化. 24 (7, 8). 369-374 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Yano et al: "Immunolocalization of lipoprotein (a) in wounded tissues." J. Histochem. Cytochem.45 (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢野容子: "血管内皮細胞の遊走および増殖に及ぼすリポ蛋白(a)、低比重および高比重リポ蛋白の影響に関する研究" 岐阜大学医学部紀要. 45 (2) (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野間昭夫: "リポ蛋白(a)のすべて" 中外医学社, 278 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Makino et al.: "Rhesus monkey lipoprotein (a). Relationship between apo (a) isoforms and Lp (a) density in plasma." Ann.N.Y.Acad.Sci.748. 603-605 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Abe et al.: "Polymorphism and distribution of apo (a)." J.Atheroscler. Thromb.2. s 8-s 12 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Arakawa et al.: "Examination of Lp (a) in the arterial wall of arteriosclerosis obliterans patients." J.Jpn.Coll.Angiol.15(6). 552-555 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Noma.: "Lipoprotein (a), Lp (a)." J.Jpn.Atheroscler. Soc.24(7,8). 369-374 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Yano et al.: "Immunolocalization of Lp (a) in wounded tissues." J.Histochem. Cytochem.45. 1-15 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Yano et al.: "Effect of Lp (a), low and high density lipoproteins on migration and proliferation of endothelial cells : b-Fibroblast growth factor-mediated mechanism." Acta Sch.Med.Univ.Gifu. 45(2). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Noma.: Lopoprotein (a). Chugai-Igakusha, 278 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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