1997 Fiscal Year Final Research Report Summary
Establishment of severe combined immune deficient mice introduced with human MDR1 geneinducted by murine endothelin promoter
| Project/Area Number |
07457588
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokai University |
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Principal Investigator |
TAMAOKI Norikazu Tokai University School of Medicine Professor, 医学部, 教授 (50055860)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Hitoshi Tokai University School of Medicine Assistant Professor, 医学部, 講師 (20191273)
NAKAMURA Masato Tokai University School of Medicine Assistant Professor, 医学部, 講師 (00164335)
UEYAMA Yoshito Tokai University School of Medicine Associate Professor, 医学部, 助教授 (30072408)
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| Project Period (FY) |
1995 – 1997
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| Keywords | brain tumor / multidrug resistance / MDR1 gene / blood brain barrier / molecular pathology / reversal of MDR / transgenic mouse / expreimental therapy |
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| Research Abstract |
Human glioma usually shows intrinsic multidrug resistance due to the blood brain barrier (BBB), in which membrane-associated P-glycoprotein (PGp) encoded by human multidrug resistance gene MDR1 plays certain roles. We established severe combined immune deficient (SCID) mice harboring murine endothelin driven human MDR1 gene. Then, we are studying drug sensitivity to vincristine (VCR), doxorubicin (DOX) and nimustine (ACNU) in subcutaneously xenotransplanted human glioma. We examined the levels of MDR1 gene expression in the xenografts by reverse transcriptase-polymerase chain reaction, and the localization of PGp by immunohistochemistry. The localization of PGp was limited to the stromal vessels in the xenografts by immunohistochemistry. The in vivo chemosensitivity test, now going, will provide us some insights for PGp expressed on the stromal vessels as a major contributing factor to multidrug resistance in human glioma.
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[Publications] Eiichiro Ota, Yoshiyuki Abe, Yoshiro Oshika, Yuichi Ozeki, Masayuki Iwasaki, Hiroshi Inoue, Hitoshi Yamazaki, Seiichi Tamai, Yoshito Ueyama, K.Takagi, Toshiro Ogata, Norikazu Tamaoki, Massato Nakamura: "Expression of the multidrug resistance-associated protein (MRP) gene in non-small cell lung cancer" British J.Cancer. 72. 550-554 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshiyuki Abe, Hitoshi Yamazaki, Yoshiro Oshika, Ryuji Suto, Atsushi Tsugu, Eiichiro Ota, Hidesuke Satoh, Yasuyuki Ohnishi, Tadashi Yanagawa, Yoshito Ueyama, Norikazu Tamaoki, Masato Nakamura: "Advantage of in vivo chemosensitivity assay to detect vincristine-resistance in a human epidermoid carcinoma xenografts" Anticncer Research. 16. 729-734 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshiyuki Abe, Yasuyuki Ohnishi, Masumi Yoshimura, Eiichiro Ota, Yuuichi Ozeki, Yoshiro Oshika, Tetuji Tokunaga, Hitoshi Yamazaki, Yoshito Ueyama, Toshiro Ogata, Norikazu Tamaoki, Masato Nakamura: "P-glycoprotein-mediated multidrug resistance of human lung cancer cell in vivo" British J.Cancer. 74. 1929-1934 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshiyuki Abe, Yoshiro Oshika, Yasuyuki Ohnishi, Ryuji Suto, Tetsuji Tokunaga, Hitoshi Yamazaki, Hiroshi Kijima, Nobuyoshi Hiraoka, Yoshito Ueyama, Norikazu Tamaoki, Masato Nakamura: "A xenograft line of human teratocarcinoma established by serial transplantation in severe combined immunodeficient (SCID) mice" APMIS. 105. 283-289 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ryuji Suto, Yoshiyuki Abe, Masato Nakamura, Yasuyuki Onishi, Masumi Yoshimura, Yoon Hwan Lee, Takashi Imanishi, Hitoshi Yamazaki, Hiroshi Kijima, Testuji Tokunaga, Yoshiro Oshika, Nobuyoshi Hiraoka, Norikazu Tamaoki, Hiroaki Fukuda, Yoshito Ueyama: "Multidrug resistance mediated by overexpression of P-glycoprotein in human osteosarcoma in vivo" International Journal of Oncology. 12. 287-291 (1998)
Description
「研究成果報告書概要(欧文)」より
