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1996 Fiscal Year Final Research Report Summary

Research on factors regulating the activation of latently infection Epstein-Barr virus

Research Project

Project/Area Number 07457589
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionHokkaido University

Principal Investigator

TAKADA Kenzo  Hokkaido University School of Medicine, Cancer Institute, Department of Virology, Professor, 医学部, 教授 (30133721)

Co-Investigator(Kenkyū-buntansha) SUGIURA Makoto  Hokkaido University School of Medicine, Cancer Institute, Department of Virology, 医学部, 助手 (20241317)
Project Period (FY) 1995 – 1996
KeywordsEpstein-Barr virus / latent infection / activation / anti-immunoglobulin antibody / promoter
Research Abstract

This study aimed to identify factors regulating the expression of the BZLF1 gene which acts as the switch for shift from Epstein-Barr virus (EBV) latent infection to virus replicative cycle. Burkitt's lymphoma-derived Akata cells have a very unique property that cells are in a latent state of EBV infection and shift to virus replicative cycle upon treatment of cells by anti-immunoglobulin antibodies (anti-Ig). Recently, we have isolated EBV-negative subclones from parental Akata cells. By using EBV-negative Akata cells, we searched for cellular genes involved in virus induction from latent infection.
Nuclear extract was isolated from anti-Ig-treated and -untreated cells and analyzed by a gel shift assay with probes specific for 3 BZLF1 promoter regions that had been shown to be important for BZLF1 expression (Shimizu et al., J.Virol.67 : 3240-3245,1993). We could see many bands, however the competition experiments revealed that none of them was specific. Further study with different conditions may allow to identify specifc bands.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yoshiyama, H.: "Persistent Epstein-Barr virus infection in a human T-cell line:unique program of latent virus expression." EMBO J.14. 3706-3711 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takada, K.: "Pathogenic role of Epstein-Barr virus in human cancer." Intervirology. 38. 214-220 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu, N.: "Clonal propagation of Epstein-Barr virus(EBV)recombinants in EBV-negative Akata cells." J.Virol. 70. 7260-7263 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wen, S.: "Association of Epstein-Barr virus(EBV)with sjogren's syndrome:differential EBV expression between epithelilal cells and lymphocytes in salivary glands." Am.J.Pathol. 149. 1511-1517 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koide J.: "Spontaneous establishment of an Epstein-Barr vitus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient." J.Virol.71. 2478-2481 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiyama H.: "Epstein-barr virus infection to human gastric carcinoma cells:implication of the existense of a new virus receptor different from CD21." J.Virol.71. 5688-5691 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiyama, H., Shimizu, N.and Takada, K.: "Persistent Epstein-Barr virus infection in a human T-cell lilne : unique program of latent virus expression." EMBO J.14. 3706-3711 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takada, K., Shimizu, N., Tanabe-Tochikura, A.and Kuroiwa, Y.: "Pathogenic role of Epstein-Barr virus in human cancer." Intervirology. 38. 214-220 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, N., Yoshiyama, H., and Takada, K.: "Clonal propagation of Epstein-Barr virus (EBV) recombinants in EBV-negative Akata cells." J.Virol.70. 7260-7263 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wen, S., Shimizu, N., Yoshiyama, H., Miyazaki, Y., Shinozaki, F.and Takada, K.: "Association of Epstein-Barr virus (EBV) with sjog ren's syndrome : differential EBV expression between epithelilal cells and lymphocytes in salivary glands." Am.J.Pathol.149. 1511-1517 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koide, J., Takada, K., Sugiura, M., Sekine, H., Ito, T., Saito, K., Mori, S., Takeuchi, T., Uchida, S and Abe, T.: "Spontaneous establishment of an Epstein-Barr vitus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient." J.Virol.71. 2478-2481 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiyama, H., Imai, S., Shimizu, N.and Takada, K.: "Epstein-barr virus infection to human gastric carcinoma cells : implication of the existense of a new virus receptor different from CD21." J.Virol.71 (7). 5688-5691 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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