• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

Systematic search for novel proteinases in gastrointestinal tract and studies on their physiological roles and structure/function

Research Project

Project/Area Number 07458149
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

TAKAHASHI Kenji  Tokyo University of Pharmacy and Life Science, Department of Life Science, Professor, 生命科学部, 教授 (70011533)

Co-Investigator(Kenkyū-buntansha) KOJIMA Masaki  Tokyo University of Pharmacy and Life Science, Department of Life Science, Assis, 生命科学部, 助手 (90277252)
INOUE Hideshi  Tokyo University of Pharmacy and Life Science, Department of Life Science, Assis, 生命科学部, 助教授 (20184765)
Project Period (FY) 1995 – 1996
Keywordsgastrointestinal proteinase / VIP-degrading endopeptidase / gastric trypsin / enteropeptidase / hepsin / pepsinogen / _2-macroglobulin / trypsin
Research Abstract

1.A novel membrane-bound serine proteinase degrading vasoactive intestinal polypeptide fairly specifically and a novel membrane-associated intracellular trypsin were isolated from porcine gastric antral mucosa and characterized.
2.Enteropeptidase cDNA was isolated and cloned from the cDNA library of rat duodenum and its primary structure was deduced. In addition, the tissue distribution
of its mRNA was analyzed.
3.A novel membrane-associated metalloproteinase degrading progastrin peptide fairly specifically was isolated from porcine brain and characterized.
4.The microsomal membrane-bound serine proteinase from rat liver was identified as hepsin from analyzes of its polypeptide chain composition and partialamino acid sequence.
5.Pepsinogen isoforms were isolated and characterized from the gastric mucosae of turtle and tuna stomach and the amino acid sequences of their major forms were determined.
6.Normal human blood alpha _2-macroglobulin was shown to contain a small but significant amount of novel serine proteinase (s) naturally entrapped in it.
7.Pancreatic trypsin and alpha-chymotrypsin were shown to selectively cleave Arg-X and Phe-X bonds, respectively, at a high alkaline pH (pH-13).
8.In relation to this project, additional characterization studies were performed on some other proteinases including follipsin, prolylendopeptidase, acid proteinase B,and signal peptidase and bromelain inhibitors.

  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Gwang-Ho Jeohn: "Purification and characterization of a Vasoattive Intestinal Polypeptide-degrading Endoprotease from Poricine Antral Mucosal Membranes" Journal of Biological Chemistry. 270. 7809-7815 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gwang-Ho Jeohn: "Isolation and Characterization of Gastric Trypsin from the Microsomal Fraction of Porcine Gastrid Antral Mucosa" Journal of Biological Chemistry. 270. 14748-14755 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junji Onishi: "Cleavage Specificity of Porcine Follipsin" Journal of Biological Chemistry. 270. 19391-19394 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ken-ichi Hatano: "Prinary Structure Sequence-specific ^1H-NMR Assignments and Secondary Structure in Solution of Bromelain Inhibitor VI Pineapple Stem" European Journal of Biochemistry. 232. 335-343 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yong-Tae Kim: "Identification of Trp 300 as an Important Residue for Escherichia coli Leader Peptidase Activity" European Journal of Biochemistry. 234. 358-362 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gwang-Ho Jeohn: "Specific Cleavage of the Gln^<15>-Lys^<16> Bond in β-Amyloid Peptidase by porcine Vasoactive Intestinal Polypeptide-degrading Endopeptidase" Protein and Peptide Letters. 3. 101-106 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naohisa Yahagi: "Complementary DNA Cloning and Sequencing of Rat Entero peptidase and Tissue Distribution of Its mRNA" Biochemical and Biophysical Research Communications. 219. 806-812 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Hirasawa: "Purification and Characterization of Turfle Pepsinogen and Pepsin" Journal of Biochemistry. 120. 407-414 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masao Tanji: "The Primary Structure of the Major Pepsinogen from the Gastric Mucosa of Tuna Stomack" Journal of Biochemistry. 120. 647-656 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuko Sakurai: "Trypsin-like Endopeptidasecs Naturally Entopped in Human Blood α_2-Macroglobulin" Biomedical Research. 17. 347-350 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takayuki Takahashi: "Prolye Endopeptidase from the Follicular Flaid of Porcine Ovary : Comparison with the Liver Enzyme" Biomedical Research. 17. 435-442 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideshi Inoue: "Heterologous Expression and Site-directed Mutagenesis Studies on the Activation Mechanism and the Roles of the Basic Residues in the Prosegment of Aspergillopepsinoger I" European Journal of Biochemistry. 237. 719-725 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ken-ichi Hatano: "Solution Structure of Bromelain Inhibitor VI Pineapple Stem : Structural Similarity with Bowman-Birk Trypsin/Chymotrypsin Inhibitor from Soybeaen" Biochemistry. 35. 5379-5384 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ken-ichi Hatano: "Tertiary Structure Comparison of Bromelain Inhibitor VI from Pineapple Sten with Bovine Pancreatic Trypsin Inhibitor" Proceedings of the Japan Academy. 72. 104-107 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hee-Jung Kim: "Purification and Characterization of an Extracellular Metalloprotease from Pseudomonas fluorescens" Journal of Biochemistry. 121. 82-88 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenji Takahashi: "The Specificity of Peptide Bond Cleavage of Acid Proteinase A from Aspergillus niger var.macrosporus toward Oxidiged Ribonuclease A" Bioscience,Biotechnology and Biochemistry. 61. 381-383 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hidemi Matsuzaki: "Evidence for the Identity of Rat Liver Microsomal Membrance-bound Serine Proteinase with Hepsin" Protein and Peptide Letters. 4(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenji Takahashi: "Restriction of the Specifications of Trypsin and α-Chynofrypsin at a High Alkaline pH Values" Protein and Peptide Letters. 4(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gwang-Ho Jcohn: "Purification and Characterization of a Vasoactive Intestinal Polypeptide-Degrading Endoprotease from Porcine Antral Mucosal Membranes" J.Biol.Chem.270. 7809-7815 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gwang-Ho Jcohn: "Isolation and Characterization of a Gastric Trypsin from the Microsomal Fraction of Porcine Antral Mucosa" J.Biol.Chem.270. 14748-14755 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junji Ohnishi: "Cleavage Specificity of Porcine Follipsin" J.Biol.Chem.270. 19391-19394 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ken-ichi Hatano: "Primary Structure, Sequence-Specific ^1H-NMR Assignments and Secondary Structure in Solution of Bromelain Inhibitor VI from Pineapple Stem" Eur.J.Biochem.232. 335-343 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yong-Tac Kim: "Identification of Trp300 as an Important Residue for Escherichia coli Leader Peptidase Activity" Eur.J.Biochem.234. 358-362 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gwan-Ho Jeohn: "Specific Cleavage of the Gln^<15>-Lys^<16> Bond in beta-Amyloid Peptides by Porcine Vasoactive Intestinal Polypeptide-degrading Endopeptidase" Protein and Peptide Lett.3. 101-106 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naohisa Yahagi: "Complementary DNA Cloning and Sequencing of Rat Enteropeptidase and Tissue Distribution of Its mRNA" Biochem.Biophys.Res.Commun.219. 806-812 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Hirasawa: "Purification and Characterization of Turtle Pepsinogen and Pepsin" J.Biochem.120. 407-414 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masao Tanji: "The Primary Structure of the Major Pepsinogen from the Gastric Mucosa of Tuna Sromach." J.Biochem.120. 647-656 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuko Sakurai: "Trypsin-like Endopeptidase (s) Naturally Entrapped in Human Blood 2-Macroglobulin" Biomed.Res.17. 347-350 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takayuki Takahashi: "Prolyl Endopeptidase from the Follicular Fluid of Porcine Ovary : Comparison with the Liver Enzyme" Biomed.Res.17. 435-442 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideshi Inoue: "Heterologous Expression and Site-Directed Mutagensis Studies on the Activation Mechanism and the Roles of the Basic Residues in the Prosegment of Aspergillopepsinogen I" Eur.J.Biochem.237. 719-725 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ken-ichi Hatano: "Solution Structure of Bromelain Inhibitor VI from Pineapple Stem : Structural Similarity with Bowman-Birk Trypsin/Chymotrypsin Inhibitor from Soybean" Biochemistry. 35. 5379-5384 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ken-ichi Hatano: "Tertiary Structure Comparison of Bromelain Inhibitor VI from Pineapple Stem with Bovine Pancreatic Trypsin Inhibitor" Proc.Japan Acad.72. 104-107 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hee-Jung Kim: "Purification and Characterization of an Extracellular Metallo-protease from Pseudomonas fluorescens" J.Biochem.121. 82-88 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kenji Takahashi: "The Specificity of Peptide Bond Cleavage of Acid Proteinase A from Aspergillus var.macrosporus toward Oxidized Ribonuclease A" Biosci.Biotech.Biochem.61. 381-383 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hidemi Matsuzaki: "Evidence for the Identity of the microsomal membrane-bound serine proteinase from rat liver as hepsin" Protein and Peptide Lett.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kenji Takahashi: "Restriction of the Specificities of Trypsin and alpha-Chymotrypsin at a High Alkaline pH Value" Protein and Peptide Lett.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi