1996 Fiscal Year Final Research Report Summary
Dynamisms and funstions of calpain in early embryogenesis
| Project/Area Number |
07458157
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
EMORI Yasufumi University of Tokyo, Graduate School of Science, Associate Professor, 大学院・理学系研究科, 助教授 (60160389)
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| Project Period (FY) |
1995 – 1996
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| Keywords | calpain / calcium / Drosophila / signalling / cytoskeleton / actin-cytoskeleton / proteclysis / early embryogenesis |
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| Research Abstract |
Changes in shapes of cells during cell division and cell differentiation are mainly dependent on actin-related cytoskeletons on the cell surface. Calpain, calcium-dependent neutral protease, has proteolytic activity which irreveribly changes the components of the cytoskeleton. In this study, the physiological functions of calpain have been analyzed by developmental and biochmical methods during early embryogenesis of Drosophila melanogaster. First, localization of calpain and actin was analyzed by immunohistochmical methods. Second, cDNA clones for actin-binding proteins, probable substrates for calpain, were obtained and expressed in Escherichia coli together with the expression of active calpain. Third, the probes for calpain reaction, specific antibodies for cleavage sites generated by calpain reaction, were prepared. Fourth, using these probes described above, dynamism of localization of calpain and its substrates were analyzed during Drosophila development. Consequently, the results were obtained that calpain is involved in the formation and reorganization of actin-related cytoskeleton in pseudo-cleavage furrow.
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Research Products
(10 results)
