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1996 Fiscal Year Final Research Report Summary

Study of ric3 related to ICE with apoptosis-driving activity

Research Project

Project/Area Number 07458180
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionOsaka University

Principal Investigator

TSUJIMOTO Yoshihide  Osaka University Medical School, Professor, 医学部, 教授 (70132735)

Co-Investigator(Kenkyū-buntansha) KAMADA Shinji  Osaka University Medical School, Assistant Professor, 医学部, 助手 (20243214)
Project Period (FY) 1995 – 1996
Keywordsapoptosis / ICE / Fas
Research Abstract

Apoptosis is an important suicide program involved in a variety of biological phenomena including morphogenesis and maintenance of tissue homeostasis. Apoptosis is an important theme not only in biology but also in medical field because dysregulation of apoptosis leads to various diseases such as cancer and Alzheimer's disease. However the molecular basis of apoptosis is still largely unknown. We have been studying ICE (interleukin 1-beta-converting enzyme) family proteases with apoptosis-driving activity. Although several ICE-related genes have been identified in mammals, it is still to be determined which member is involved in apoptosis.
We focused on two ICE-related genes, ric2 and ric3, both of which are most closely related to ICE itself. We have shown using a dominant negative mutant and specific antibodies that ric2 is involved in Fas-mediated apoptosis, providing the first evidence for an essential role of ric2 in apoptotic cell death. We have also shown that proteolytic activity of ric2 and ric3 gene products are inhibited by CrmA which is initially identified as an ICE inhibitor and has recently been shown to inhibit many forms of apoptosis, suggesting that both ric2 and ric3 play an important role in apoptosis.
Since elements with apoptosis-inducing scitivity might be very useful for treatment of cancers, these date would be useful not only for understanding molecular basis of apoptosis but also for control of neoplastic diseases.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] J. Hasegawa: "Involvement of CPP32/Yama (-like) proteases in Fas-mediated apoptosis" cancer Research. 56. 1713-1718 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Shigenaga: "Targeted expression of ced-3 and Ice induces programmed cell death in Drosophila" Cell Death & Differentiation. (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Shimizu, et al.: "Prevention of hypoxia-induced cell death by Bcl-2 and Bcl-xL." Nature. 374. 811-813 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Suzuki, et al.: "Involvement of Fas in regression of vaginal epithelia after ovariectomy and during an estrous cycle." EMBO J.15. 211-215 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hasegawa, J.-I., Kamada, S., Kamiike, W., Shimizu, S., Imazu, T., Matsuda, H.and Tsujimoto, Y.: "Involvement of CPP32/Yama (-like) proteases in Fas-mediated apoptosis" Cancer Res.56. 1713-1718 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigenaga, A., Funahashi, Y., Kimura, K., Kobayakawa, Y., Kamada, S., Tsujimoto, Y.and Tanimura, T.: "Targeted expression of ced-3 and Ice induce programd cell death in Drosophila" Cell Death & Differentiation. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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