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1996 Fiscal Year Final Research Report Summary

STUDY ON TRANSCRIPTION FACTORS REGULATING GENE EXPRESSION IN SEA URCHIN EMBRYOS

Research Project

Project/Area Number 07458195
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

SHIMADA Hiraku  HIROSHIMA UNIVERSITY,FACULTY OF SCIENCE,PROFESSOR, 理学部, 教授 (70011559)

Co-Investigator(Kenkyū-buntansha) NAKATSUBO Keiko  HIROSHIMA UNIVERSITY,FACULTY OF SCIENCE,INSTRUCTOR, 理学部, 助手 (40192760)
AKASAKA Koji  HIROSHIMA UNIVERSITY,FACULTY OF SCIENCE,ASSOCIATE PROFESSOR, 理学部, 助教授 (60150968)
Project Period (FY) 1995 – 1996
Keywordssea urchin / arylsulfatase / gene expression / embryo / enhancer / 0tx / insulator
Research Abstract

The present research project has been undertaken to elucidate a cascade of transcription factors related to temporally and spatially regulated expression of genes during early development of sea urchin. Results obtained during the research period for this grant are as follows.
(1) A procedure to introduce exogenous DNA into sea urchin eggs by a particle gun has been developed.
(2) Temporal expression of the Ars gene is regulated by two distinct cisactive elements in its promoter region (from -252 to +38bp), though level of transcription initiated by these elements alone is very low and declines rapidly.
(3) GGATTA sequences (known as binding sites for transcription factor 0tx) in the first intron of the Ars gene act as a transcriptional enhancer of this gene. Two distinct cDNAs (Hp0tx_E and Hp0tx_L) encoding Hp0tx have been cloned. Hp0tx_E is a maternal factor and suppresses the Ars gene transcription, while Hp0tx_L is produced in hatched blastulae and activates the Ars gene transcription. Two cDNAs are produced from the same gene by alternative splicing.
(4) It is found that the region from -2686 to -2113bp of the Ars gene acts as an transcriptional insulator that prevents the Ars gene to be transcribed under the influence of promoter of the gene located in a closely vicinity.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Sakamoto et al.: "A triplex DNA configuration of the polypyrimldine : polypurine stretch in the 5' flanking region of the sea urchin arylsulfatase gene." Zoological Science. 13. 105-109 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada et al: "Spatial expression of a forkhead homologue in the sea urchin embryo." Mechanism of Development. 60. 163-173 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsunaga-Nakatsubo et al: "cDNA cloning of Na_+,K_+-ATPase alpha subunit from embryos of the sea urchin,Hemicentrotus pulcherrimus." Zoological Science. 13. 833-841 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakamoto et al: "Two isoforms of orthodenticle-related proteins (HpOtx) bind to the enhancer element of sea urchin arylsulfatase gene." Developmental Biology. 181. 284-295 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akasaka et al: "Oral-aboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium ionophore." Development Growth and Differentiation. 39(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morokuma et al: "A cis-regulatory element within the 5' flanking region of arylsulfatase gene of sea urchin,Hemicentrotus pulcherrimus" Development Growth and Differentiation. 39(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Sakamoto, K.Akasaka, T.Yamamoto and H.Shimada: "A triplex DNA configuration of the polypyrimidine : polypurine stretch in the 5' flanking region of the sea urchin arylsulfatase gene." Zool.Sci.13. 105-109 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Harada, K.Akasaka, H.Shimada, K.J.Peterson, E.H.Davidson and N.Satoh: "Spatial expression of a forkheao homologue in the sea urchin embryo." Mech.Dev.60. 163-173 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mitsunaga-Nakatsubo, K.Yamazaki, M.Hatoh-Okazaki, H.Kawashita, C.Okamura, K.Akasaka, H.Shimada and I.Yasumasu: "cDNA cloning of Na+, K+-ATPase alpha subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus." Zool.Sci.13. 833-841 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Sakamoto, K.Akasaka, K.Mitsunaga-Nakatsubo, K.Takata, T.Nishitani and H.Shimada: "Two isoforms of orthodenticlerelated proteins (Hp0tx) bind to the enhancer element of sea urchin arylsulfatase gene." Dev.Biol.181. 284-295 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Akasaka, H.Uemoto, F.Wilt, K.Mitsunaga-Nakatsubo and H.Shimada: "Oral-aboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium ionophore." Develop.Growth & Differ. 39(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Morokuma, K.Akasaka, K.Mitsunaga-Nakatsubo and H.Shimada: "A cis-regulatory element within the 5' flanking region of arylsulfatase gene of sea urchin, Hemicentrotus pulcherrimus." Develop.Growth & Differ. 39(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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