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1997 Fiscal Year Final Research Report Summary

Isolation and characterization of the genes regulating the neuronal defferentiation.

Research Project

Project/Area Number 07458210
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNational institute of Neuroscience

Principal Investigator

MOMOI Takashi  National institute of Neuroscience, Division of Developmental and Differentiation, Room Chief, 神経研究所疾病第5部, 室長 (40143507)

Project Period (FY) 1995 – 1997
KeywordsRetinoic acid / P19EC / Oligossachryltransferase / neuronal cell differentiation / sonic hedgeho / spermatocyto
Research Abstract

P19EC cells differentiate into neuronal cells by retinoic acid. During neuronal differentiation, many cells undergo apoptosis with DNA fragmentatoin. Thus neuronal differentiation of P19EC cells is a good model for the neuronal differentiation and apoptosis during development of mouse embryos. In the present study, we isolated various genes expressed during neruonal differentiation of P10EC cells induced by retinoic acid. 1) Caspase-3 and -7, which are involved in the apoptosis of P19EC cells induced by retinoic acid. 2) Oligosaccharyltransferase 48kDa, whose defect induces the death of yeast. 3) RA70, a retinoic acid inducible new gene, which is expressed in the nervous system of the early developmental stage of mouse embryos. 4) RA175, a new gene, which is expressed in epithelial tissues and nervous system during neurogenesis. Since RA175 is coexpressed in sonic hedgehog in epithelial tissues, RA175 may be involved in the morphogenesis induced by sonic hedgehog-patched signal. 5) RA375, which is the gene with zinc-finger motif expressed in the nervous sytem. In extra-neural tissues, RA70 is expressed in spermatogonia, but not spermatid during spermatogenesis, suggesting that RA70 is also involved in the meiosis.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Fujita, E.: "Wortmannin enhances acirvation of CPP32(Capase-3)induced by TNF or anti-Fas." Cell Death and Differentiation. In press (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita, E.: "Involvement of Sonic hedgehog in the cell growth of LK-2 cells,human lung squamous carcinoma cells." Biocem.Biophys.Res.Commun.238. 658-664 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamagata, T.: "Genome organization of human 48kDa oligossachryltransferase" Genomics. 45. 535-540 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mukasa, T.: "Specific expression of CPP32 in sensory neurons of mouse embryos and activation of CPP32 in the apoptosis induced by a withdrawal of NGF." Biochem.Biophys.Res.Commun.231. 770-774 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mukasa, T.: "Wortmannin enhances CPP32-like activity during neuronal differentiation of P19EC cells induced by retinoic acid." Biocem.Biophys.Res.Commun.232. 192-197 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita, E., Kouroku, Y., Miho, Y., Tsukahara, T., Ishiura, S.and Momoi, T.: "Wortmannin enhances activation of CPP32 (Caspase-3) induced by TNF or anti-Fas" Cell Death and Differentiation. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujita, E.Kouroku, Y., Urase, K., Tsukkahara, T., Momoi, M.Y., Kumagai, H., Takemura, T., Kuroki, T.and Momoi, T.: "Involvement of Sonic hedgehog in the cell growth of LK-2 cells, human lung sqamous carcinoma cells." Biochem.Biophys, Res.Commun. 238. 658-664 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamagata, T., Tsuru, T., Momoi, M.Y., Suwa, K., Nozaki, Y., Mukasa, T., Ohashi, H., Fukushima, Y., and Momoi, T.: "Genome organization of human 48kDa oligossachryltransferase" Genomics. 45. 535-540 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mukawa, T., Kouroku, Y., Tsukahara, T., , Momoi, Y.M., Kimura, I., and Momoi, T.: "Wortmannin enhances CPP32-like activity during neuronal differentiation of P19EC cells induced by retinoic acid" Biochem.Biphys.Res.Commun. 232. 192-197 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mukasa, T., Urase, K., Momoi, Y.M., Kimura, I., and Momoi, T.: "Specific expression of CPP32 in sensory neurons of mouse embryos and activation of CPP32 in the apoptosis induced bya withdrawal of NGF" Biochem.Biophys.Res.Commun. 231. 770-774 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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