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1996 Fiscal Year Final Research Report Summary

STUDY OF A NEURON-SPECIFIC PROTEIN,HPC-1, WHICH REGULATES NEURONAL SPROUTING.

Research Project

Project/Area Number 07458214
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionKYORIN UNIVERSITY

Principal Investigator

AKAGAWA Kimio  KYORIN UNIVERSITY,SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (80129303)

Co-Investigator(Kenkyū-buntansha) OSANAI Makoto  KYORIN UNIVERSITY,SCHOOL OF MEDICINE,INSTRUCTOR, 医学部, 助手 (90286419)
FUJIWARA Tomonori  KYORIN UNIVERSITY,SCHOOL OF MEDICINE,INSTRUCTOR, 医学部, 助手 (90255399)
FUJINO Ichiro  KYORIN UNIVERSITY,SCHOOL OF MEDICINE,INSTRUCTOR, 医学部, 助手 (30265764)
YAMAGUCHI Kazuhiko  KYORIN UNIVERSITY,SCHOOL OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (00191221)
Project Period (FY) 1995 – 1996
KeywordsHPC-1 / SYNTAXIN 1A / EXOCYTOSIS / NEURITE SPROUTING / REGULATED SECRETION
Research Abstract

This research project was carried out in order to study the physiological role of HPC-1/Syntaxin 1A.Administration of the antibody against HPC-1 resulted in increase of catechoilamine release from PC12 cells and transmitter release from autosynapse of cultured hippocampal neurons. Overexpression of HPC-1 in beta TC cell line caused decrease of insulin secretion. These results suggested that HPC-1 playd important role in regulated secratory process as a negative regulator. In dorsal root ganglion cells and cerebellar granular cells in vitro, administration of the antisense oligonucleotide against HPC-1 mRNA enhanced neurite sprouting within 12 hours. Transfection experiments with a vector carrying the antisense RNA to PC12 cells showed that suppression of HPC-1 expression caused increase of catecholamin release and enhancement of small process formation, clearly indicating the multifunctional role of HPC-1. Expression of HPC-1 gene in hippocampal neurons was suppressed by kainic acid.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] S.Nagamatsu: "Expression and functional role of syntaxin 1/HPC-1 in pancreatic β-cells." Joural of Biological Chemistry. 271. 1160-1165 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamaguchi: "Enhancement of neurite sprouting by suppression of HPC-1/syntaxin activity in the cultured verfebrate nerve cells." Brain Research. 740. 185-192 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Kushima: "Chara cterization of HPC-1 antigen,an isoform of syntaxin 1,with the isoform specific monoclonal antibody,14D8." Joural of Molecular Neuroscience. (in Press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Fujiwara: "In teraction of HPC-1/syntaxin 1A with the cytoskeletal protein,tubulin." Biochemical and Biophysical Research Communication. (in Press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Nakayama: "Mapping of human HPC-1/syntaxin 1A gene to chromosome 7 bandq 11.2" Genomics. (in Press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Nagamatsu, T.Fujiwara, Y.Nakamichi, T.Watanabe, H.Sawa and K.Akagawa: "Expression and functional role of syntaxin 1/HPC-1 in pancreatic beta Cells : Syntaxin 1A but not 1B play a negative role in regulatory insulin relaesepathway" J.Biol.Chem.271. 1160-1165 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaguchi, T.Nakayama, T.Fujiwara and K.Akagawa: "Enhancement of neurite sprouting by suppression of HPC-1/syntaxin 1A activityin the cultured vertebrate nerve cells." Brain Res.740. 185-192 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F.Miya, A.Yamamoto, K.Akagawa, K.Kawamoto & Y.Tashiro: "Localization of the HPC-1/syntaxin 1A in developing rat cerebellar cortex." Cell Structure and Function. 21. 525-532 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Kushima, T.Fujiwara, M.Sanada and K.Akagawa: "Characterization of HPC-1 antigen, an isoform of syntaxin 1, with the isoform-specific monoclonal antibody, 14D8" J.Mol.Neurosci.8. 19-27 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Fujiwara, K.Yamaguchi, T.Yamamori and K.Akagawa: "Interaction of HPC-1/syntaxin 1A with the cytoskeletal protein, tubulin." Biochem.Biophys.Res.Comm.231. 352-355 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Nakayama, T.Fujiwara, A.Miyazawa, S.Asakawa, N.Shimizu, Y.Shimizu, K.Mikoshiba and K.Akagawa: "Mapping of human HPC-1/syntaxin 1A gene to chromosome 7 band q11.2." Genomics. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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