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1996 Fiscal Year Final Research Report Summary

Steroid receptors-multiforms and their significance on brain differentiation

Research Project

Project/Area Number 07458257
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

KAWATA Mitsuhiro  Department of Medicine, Kyoto Prefectural University of Medicine Professer, 医学部, 教授 (60112512)

Co-Investigator(Kenkyū-buntansha) YOKOYAMA Keiko  Department of Medicine, Kyoto Prefectural University of Medicine Assistant, 医学部, 助手 (70264752)
MORITA Noriyuki  Department of Medicine, Kyoto Prefectural University of Medicine Assistant, 医学部, 助手 (50239662)
OZAWA Hitoshi  Department of Medicine, Kyoto Prefectural University of Medicine Lecturer, 医学部, 講師 (60169290)
Project Period (FY) 1995 – 1996
Keywordssteroid hormone / receptor / immunohistochemistry / in situ hybridization / brain / apoptosis / cytoskeleton / plasticity
Research Abstract

The function of the N-terminal domain of steroid hormone receptors is not fully understood, and appears to modulate transcription. Antibodies to steroid hormone receptors are generally directed against the N-terminal region because of its strong hydrophilicity. Estrogen receptor immunoreactivity was localized in the nuclei of neurons, not in the cytoplasm or non-neuronal cells (glia, endothelium, and ependymal cells) in the ovariectomized rat. The medial preoptic area of female rat contains more neurons containing the estrogen receptor immunoreactivity than male. In the anteroventral periventricular nucleus of the preoptic area (AVPN-POA), estrogen receptor immunoreactive neurons are present in numbers several times higher in females than males. Estrogen treatment of ovariectomized rats reduces the number of estrogen receptor-immunoreactive neurons in the AVPN-POA of females. It was found that neurons extending their axons and dendrites contain estrogen receptors. Whether axons or dendrites are specifically affected by gonadal steroids is still controversial, because hypothalamic neurons have no distinctive polarity such as neurons in the hippocampus. The number of cells containing glucocorticoid receptor immunoreactivity and the intensity of immunoreactivity per cell showed age-related variations. Immunohistochemical studies using an antibody against rat liver glucocorticoid receptor showed that overall intensity of glucocorticoid receptor immunoreactivity in the brain was the highest perinatally, decreased to a low intensity around postnatal day 12, and thereafter increased gradually again to moderate staining in most brain areas. Dexamethasone treatment at the neonatal period induces apoptotic changes of granule cells in the dentate gyrus. This suggests that adrenal steroids have an indirect influence on cell birth through an as yet unidentified mechanism.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kawata,M.: "Roles of steroid hormones and their receptors in structural organization in the nervous system" Neurosci.Res.24. 1-46 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Pasterkamp,R.J.et.al.: "The perinatal ontogeny of estrogen receptor-immunoreactivity in the developing male and female rat hypothalamus" Dev.Brain Res.91. 300-303 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hu,Z.et.al.: "Exposure of postnatal rats to glucocorticoids suppresses the development of choline acetyltransferase-immunoreactive neurons:role of adrenal steroids in the development of forebrain cholinergic neurons" J.Chem.Neuroanat.10. 1-10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morimoto,M.et.al.: "Phenotypic alterations of neuropeptide Y,vasoactive intestinal peptide and choline acetyltransferase in rat cultured cromaffin cells as effected by nerve growth factor and glucocorticoid" Arch.Histol.Cytol.59. 205-218 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Visser,D.T.M.et.al.: "The alteration of glucocorticoid receptor-immunoreactivity in the rat forebrain following short-term and long-term adrenalectomy." Brain Res.729. 216-222 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morimoto,M.et.al.: "Distribution of glucocorticoid receptor immunoreactivity and mRNA in the rat brain:an immunohistochemical and in situ hybridization study." Neurosci.Res.26. 235-269 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河田光博 他: "バイオマニュアルUPシリーズ、脳・神経研究プロトコール" 羊土社, 231 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森本昌史 他: "組織細胞化学1995" 学際企画, 198 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawata, M.: "Roles of steroid hormones and their receptors in structural organization in the nervous system" Neurosci. Res.24. 1-46 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Pasterkamp R.J.. et. al.: "The perinatal ontogeny of estrogen receptor-immunoreactivity in the developing male and female rat hypothalamus" Dev. Brain Res.91. 300-303 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hu, Z.et. al.: "Exposure of postnatal rats to glucocorticoids suppresses the development of choline acetyltransferase-immunoreactive neurons : role of adrenal steroids in the development of forebrain cholinergic neurons" J.Chem. Neuroanat.10. 1-10 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morimoto, M.et. al: "Phenotypic alterations of neuropeptide Y,vasoactive intestinal peptide and choline acetyltransferase in rat cultured cromaffin cells as effected by nerve growth factor and glucocorticoid" Arch. Histol. Cytol.59. 205-218 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Visser, D.T.M.et. al: "The alteration of glucocorticoid receptor-immunoreactivity in the rat forebrain following short-term and long-term adrenalectomy" Brain Res.729. 216-222 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morimoto, M.et. al: "Distribution of glucocorticoid receptor immunoreactivity and mRNA in the rat brain : an immunohistochemical and in situ hybridization study." Neurosci. Res.26. 235-269 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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