1997 Fiscal Year Annual Research Report

分子進化学的方法論に基づく遺伝子機能予測法の開発

Research Project

Project/Area Number	07504009
Research Institution	National Institute of Genetics
Principal Investigator	五條堀孝国立遺伝学研究所, 生命情報研究センター, 教授 (50162136)
Co-Investigator(Kenkyū-buntansha)	今西規国立遺伝学研究所, 生命情報研究センター, 助手 (80270461) 池尾一穂国立遺伝学研究所, 生命情報研究センター, 助手 (20249949) 舘野義男国立遺伝学研究所, 生命情報研究センター, 教授 (00202424) 池村淑道国立遺伝学研究所, 集団遺伝研究系, 教授 (50025475) 宮田隆京都大学, 理学部, 教授 (20022692)
Keywords	分子進化 / 遺伝子機能予測 / 機能的ドメイン / 分子系統樹 / セリンプロテアーゼ / MHC / 塩基配列 / シーケンシング
Research Abstract	本研究は、分子進化学、計算機科学、そして情報処理技術の最先端の研究を取り入れて、大量の配列データより抽出した配列モチーフを用いた遺伝子機能予測のためのモチーフ・データベース及び機能予測のための方法を研究開発することを目的としている。本年度は、前年度にほぼ完成させた配列モチーフ・データベースの自動更新システムの確立とそのために要する計算時間の短縮などの改良を行った。とくに並列ベクトル計算機の活用により、この改良は成功した。また、配列モチーフ抽出に不足しているDNA配列を実験的に決定するための実験基盤技術、特にPCR法のルーチン化をほぼ達成した。しかし、これらの配列モチーフの生物学的機能や構造との対応は、セリンプロテアーゼやMHCタンパクなどの一部を除いて、十分にはできなかった。一方、配列モチーフから遺伝子の分子進化や機能的ドメインのモザイク構造に関する考察は十分に達成でき、その際に用いた分子系統樹とともに、遺伝子機能予測を行うための基礎データを提供できたものと考える。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Tateno Y.: "Evolutionary motif and its biological and structural significance." J.Mol.Evol.44・1. 38-43 (1997)
[Publications] Watanabe H.: "Genome plasticity as a paradigm of eubacteria evolution." J.Mol.Evol.44・1. 57-64 (1997)
[Publications] Kurihara T.: "Molecular evolution of myelin proteolipid protein." Biochemical and Biophysical Research Communications.237. 559-561 (1997)
[Publications] Watanabe H.: "Bacterial features in the genome of methanococcus jannaschii in terms of gene composition and biased base composition in ORFs and their surrounding regions." GENE. 205. 7-18 (1997)
[Publications] Endo T.: "Evolutionary significance of intra-genome duplications on human chromosomes" GENE. 205. 9-27 (1997)
[Publications] Sato S.: "Ascidian tyrosinase gene:Its unique structure and expression in the developing brain." Developmental Dynamics. 208. 363-374 (1997)
[Publications] Suzuki Y.: "The origin and evolution of human T-Cell lymphotrophic virus type I and II" Virus Genes. 16・1. 69-84 (1998)
[Publications] Perrie reG.: "The NRSub database:update 1997." Nucl.Acids Res.25・1. 53-56 (1997)
[Publications] Nakamura Y.: "Codon usage tabulated from the international DNA sequence databases." Nucl.Acids Res.25・1. 244-245 (1997)
[Publications] Mizokami M.: "Constrained evolution of hepatitis B virus with overlapping genes." J.Mol.Evol.44・1. 83-90 (1997)
[Publications] Yamaguchi Y.: "Evolutionary mechanisms and population dynamics of the third variable envelope region of HIV within single hosts." Proc.Natl.Acad.Sci.U.S.A. 94. 1264-1269 (1997)
[Publications] Tenzen T.: "Precise switching of DNA replication timing in the GC content transition area in the human Major Histocompatibility Complex." Mol.and Celular Biology. 17・7. 4043-4050 (1997)
[Publications] Suzuki Y.: "The origin and evolution of ebola and marburg viruses." Mol.Biol.Evol.14・8. 800-806 (1997)
[Publications] Kurihara T.: "Molecular evolution of myelin proteolipid protein." Biochemical and Biophysical Research Communications.237. 559-561 (1997)
[Publications] Mizuki N.: "Major histocompatibility complex class II allelles in Kazak and Han populations in the Silk Route of northwestern China." Tissue Antigens. 50. 527-534 (1997)
[Publications] Okamoto Y.: "Father-to-mother-to-infant transmission of HIV-1:clonally transmitted isolate of infant mutates more rapidly than that of the mother and rapidly loses reactivity with neutralizing antibody." Microbiol Immunol. 41・2. 131-138 (1997)

1997 Fiscal Year Annual Research Report

分子進化学的方法論に基づく遺伝子機能予測法の開発

Principal Investigator

五條堀 孝 国立遺伝学研究所, 生命情報研究センター, 教授 (50162136)

Research Products

[Publications] Tateno Y.: "Evolutionary motif and its biological and structural significance." J.Mol.Evol.44・1. 38-43 (1997)

[Publications] Watanabe H.: "Genome plasticity as a paradigm of eubacteria evolution." J.Mol.Evol.44・1. 57-64 (1997)

[Publications] Kurihara T.: "Molecular evolution of myelin proteolipid protein." Biochemical and Biophysical Research Communications.237. 559-561 (1997)

[Publications] Watanabe H.: "Bacterial features in the genome of methanococcus jannaschii in terms of gene composition and biased base composition in ORFs and their surrounding regions." GENE. 205. 7-18 (1997)

[Publications] Endo T.: "Evolutionary significance of intra-genome duplications on human chromosomes" GENE. 205. 9-27 (1997)

[Publications] Sato S.: "Ascidian tyrosinase gene:Its unique structure and expression in the developing brain." Developmental Dynamics. 208. 363-374 (1997)

[Publications] Suzuki Y.: "The origin and evolution of human T-Cell lymphotrophic virus type I and II" Virus Genes. 16・1. 69-84 (1998)

[Publications] Perrie reG.: "The NRSub database:update 1997." Nucl.Acids Res.25・1. 53-56 (1997)

[Publications] Nakamura Y.: "Codon usage tabulated from the international DNA sequence databases." Nucl.Acids Res.25・1. 244-245 (1997)

[Publications] Mizokami M.: "Constrained evolution of hepatitis B virus with overlapping genes." J.Mol.Evol.44・1. 83-90 (1997)

[Publications] Yamaguchi Y.: "Evolutionary mechanisms and population dynamics of the third variable envelope region of HIV within single hosts." Proc.Natl.Acad.Sci.U.S.A. 94. 1264-1269 (1997)

[Publications] Tenzen T.: "Precise switching of DNA replication timing in the GC content transition area in the human Major Histocompatibility Complex." Mol.and Celular Biology. 17・7. 4043-4050 (1997)

[Publications] Suzuki Y.: "The origin and evolution of ebola and marburg viruses." Mol.Biol.Evol.14・8. 800-806 (1997)

[Publications] Kurihara T.: "Molecular evolution of myelin proteolipid protein." Biochemical and Biophysical Research Communications.237. 559-561 (1997)

[Publications] Mizuki N.: "Major histocompatibility complex class II allelles in Kazak and Han populations in the Silk Route of northwestern China." Tissue Antigens. 50. 527-534 (1997)

[Publications] Okamoto Y.: "Father-to-mother-to-infant transmission of HIV-1:clonally transmitted isolate of infant mutates more rapidly than that of the mother and rapidly loses reactivity with neutralizing antibody." Microbiol Immunol. 41・2. 131-138 (1997)

五條堀孝国立遺伝学研究所, 生命情報研究センター, 教授 (50162136)