1995 Fiscal Year Final Research Report Summary
Deparment of vaccine for new type of Vibrio cholerae O139 Bengal
| Project/Area Number |
07507001
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Research Institute, International Medical Center of Japan |
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Principal Investigator |
TAKEDA Yoshifumi International Medical Center of Japan, Research Institute, Director-General, 研究所, 所長 (30029772)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASAKI Shinji International Medical Center of Japan, Research Institute, Division Chief, 研究所, 室長 (70221653)
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| Project Period (FY) |
1995 – 1997
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| Keywords | New cholera Vibrio / O139 Bengal / Vaccine / Co60 irradiation |
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| Research Abstract |
A new strain of Vibrio cholerae O139 Bengal was first discovered in India in 1992 and a recurrence of pandemic of cholera due to the new cholera Vibrio occurred in India since 1996. To prevend pandemics of the diseases development of vaccine is most effective.
(1) Department of a candidate live vaccine : A mutant of V.cholera O139 that lacks genes for V.cholera O139 that lacks genes for cholera toxin, zonula occludens toxin, accessory cholera toxin, and Eltor hemolysin.
(2) Development of a candidate killed vaccine : V.cholerae O139 was killed by Co 60 irradiation The killed cells moved with flagella and adhered effectively to M cells.
(3) For an adjuvant to raise effective mucosal immunity, active center of A submit of cholera toxin was inactivated by replacing responsible amino acids. The mutant cholera toxin with the nontoxic A submit exerted an effective adjuvant activity by stimulating Th1 cells.
(4) Genes responsible for synthesizing 0139 LPS were analyzed.
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[Publications] C.Sharma, M.Thungapathra, A.Ghosh, A.K.Mukhopadhyay, A.Basu, R.Mitra, I..Basu, S.K.Bhattacharya, T.Shimada, T.Ramamurthy, T.Takeda, S.Yamasaki and G.B.Nair: "Molecular analysis of non-O139 Vibrio cholerae Associated with an unnusual upsurge in the Incidence of cholera-like disease in Culcutta, India"J.Clin.Microbiol. 36. 756-763 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] A.Basu, A.K.Mukhopadhyay, C.Sharma, J.Jyot, N, Gupta, A.Ghosh, S.K.Bhattacharya, Y.Takeda, A.S.G.Faruquwe M.J.Albert and G.B.Nair: "Heterogeneity in the organization of the CTX Genetic element in strains of Vibrio cholerae O139 Bengal isolated from Culcutta, India and Dhaka Bangladesh and its possible link to the Dissimilar incidence of O139 cholera in the two Locales."Microb. Pathog.. 24. 175-183 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] C.Sharma, M.Thungapathra, A.Ghosh, A.K.Mukhopadhyay, A.Basu, R.Mitra, I.Basu, S.K. Bhattacharya, T.Shimada, T.Ramamurthy, T.Takeda, S.Yamasaki and G.B.Nair: "Molecular analysis of non-O139 Vobrio cholerae Associated with an unusual upsurge in the Incidence of cholera-like diease in Culcutta, India"J.Clin. Microbiol.. 36. 756-763 (1998)
Description
「研究成果報告書概要(欧文)」より
