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1997 Fiscal Year Final Research Report Summary

Development of transposon-vector-systems in insects.

Research Project

Project/Area Number 07554067
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 遺伝
Research InstitutionKYUSYU UNIVERSITY

Principal Investigator

NITASAKA Eiji  Kyushu Univ, Biology, Research Associate, 理学部, 助手 (60222189)

Co-Investigator(Kenkyū-buntansha) OTSUKA Yasushi  Oita Medical Univ., Medical Zoology, Research Associate, 医動物学研究室, 助手 (00244161)
YUKUHIRO Kenji  NISES,Insect Genetics and Breeding, Chief Researcher, 蚕糸研究所, 主任研究官
Project Period (FY) 1995 – 1997
KeywordsTransposon / Drosophila / Bombyx mori / Mosquito / Vcctor / Transformation
Research Abstract

Transformation techniques are most important to analyze gene functions in model organisms, e. g. a mutant is transformed with a wild-type gene or a modified one. In insects, effective method for transformation had not been developed except for Drosophila. Using a transposable element, P-element, Drosophila can be transformed with high efficiency. Another advantage of this method is that tha most transgene is inserted with single copy and it eases to analyze given gene functions.
The aim of this project is to develop transformation system using transposable elements. The materials used in this study are : Drosophila melanogaster, a mosquito species (Aedes albopictus) and the silkworm (Bombyx mori). Some species of mosquito are known as vectors for diseases, e. g. malaria, dengue. The silk worm is a useful insect not only for the textiles but also for the production of biomolecules from fibroin-gland. Therefore, an effetive transformation systems were desired in these species.
Electroporat … More ion method is now widely used to transform organisms from bacteria to mammals. We tried to aply this method to insects using Drosophila embryos. In preliminary experiment with a cloned alpha-amylase gene as a reporter gene, the amylase activity was detected in the third instar larvae. Using P-element vector and a helper, however, no transformant was obtained out of 3000 embryos.
To apply an eye color gene as a transformation marker, eye color mutations of Aedes albopictus were induced by Ethyl methan sulfonate (EMS). Until now, two mutations were identified. One is brown eye1 located on the sex-chromosome and anther is brown eye2 located on an autosome.
Bm1 is a kind of SINE element of Bombyx mori. We had determined the complete sequences of fifteen of complete Bm1.1 elements. Sequence comparisons and a phylogenetic tree showed that they are classified into three groups. These results suggest that Bm1 was originated from tree ancestral elements and dispersed in the genome by reverse-transcription. Less

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Sezutsu, H.: "Evolution of the LINE-like retrotransposon I element in the Drosophila melanogaster species subgroup." Molecular and General Genetics. 249. 168-178 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nitasaka, E.: "New alleles of eye color mutations isolated from natural populations in Drosophila melanogaster." Drosophila Information Service. 76. 79 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nitasaka, E.: "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosphila melanogaster." Molecular and General Genetics. 247. 164-168 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takaoka, H: "Second case of zoonotic Onchocerca infection in a resident of Oita in Japan." Parasite. 3. 179-182 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukuhiro, K: "Preferential codon usage and two types of repetitive motifs in the fibroin gene of Chinese oak silkworm,Antheraea pernyi." Insect.Molecular Biology. 6. 89-96 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagaraju, J: "Attempt at transgenesis of the silkworm(Bombyx mori L.)by egg-injection of foreign DNA." Appl.Entomol.Zool. 31. 587-596 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Richer, B.: "Nucleotide variation and conservation at the dpp locus,a gene controlling earlydevelopment in Drosophila." Genetics. 145. 311-323 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukuhiro, K: "Preferential codon usage and two types of repetitive motifs of Chinese oak silkworm,Antheraea pernyi fibroin gene." Insect Molecular Biology. 6. 89-95 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otsuka, Y: "Genetic variation in the expression of the six hsp genes in the presence of heat shock in Drosophila melanogaster." Genes & Genetic Systems. 72. 19-24 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otsuka, Y: "Elimination of Wolbachia pipientis from Aedes albopictus." Med.Entomol.Zool. 48. 257-260 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nitasaka, E: "Current Topics on Molecular Evolution" Nei,M.and Takahata,N., 260 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sezutsu, H.: "Evolution of the LINE-like retrotransposon I element in the Drosophila melanogaster species subgroup." Mol. Gen. Gen.249. 168-178 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nitasaka, E.: "New alleles of eye color mutations lsolated from natural populations in Drosophila melanogaster." Drosophila Information Service. 76. 79 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nitasaka, E.: "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosphila melanogaster." Molecular & General Genetics. 247. 164-168 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takaoka, H.: "Second case of zoonotic Onchocerca infection in a resident of Oita in Japan." Parasite. 3. 179-182 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yukuhiro, K.: "Preferential codon usage and two types of repetitive motifs in teh fibroin gene of Chinese oak silkworm, Antheraea pernyi" Ins.Mol.Biol.6. 89-96 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagaraju, J.: "Attempt at transgenesis of the silkworm (Bombyx mori L.) by egg-injection of foreign DNA." Appl.Entomol.Zool.31. 587-596 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Richer, B.: "Nucleotide variation and conservation at the dpp locus, a gene controlling early development in Drosoophila." Genetics. 145. 311-323 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yukuhiro, K.: "Preferential codon usage and two types of repetitive motifs of Chinese oak silkworm, Antheraea pernyi fibroin gene." Ins.Mol.Biol.6. 89-95 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otsuka, Y.: "Genetic variation in the expression of the six hsp genes in the presence of heat shock in Drosophila melanogaster." Genes & Genetic Systems. 72. 19-24 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otsuka, Y.: "Elimination of Wolbachia pipientis from Aedes albopictus." Med.Entomol.Zool. 48. 257-260 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nitasaka, E.: Current Topics on Molecular Evolution. Nei, M.and Takahata, N., 260 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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