1997 Fiscal Year Final Research Report Summary
Preparations and applications of novel copolymeric adsorbents for selective removal of endotoxin
| Project/Area Number |
07555294
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
高分子合成
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| Research Institution | Kumamoto University |
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Principal Investigator |
HIRAYAMA Chuichi Kumamoto University, Applied Chemistry, Professor, 工学部, 教授 (10040429)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Sunao Kohjin Co., Ltd., Fiber & Pulp Division, General Manager, 開発技術本部, 部長
SAKATA Masayo Kumamoto University, Applied Chemistry, Assistant Professor, 工学部, 助手 (60187391)
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| Project Period (FY) |
1995 – 1997
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| Keywords | endotoxin / lipopolysaccharide / N,N-dimethylaminopropylacrylamide / divinylbenzene / bovine serum albumin / Escherichia coli / amino-group content / molecular mass exclusion |
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| Research Abstract |
Novel copolymeric adsorbents for selective removal of endotoxin from an acidic protein solution were prepared. The adsorbents comprise spherical copolymers derived from N,N-dimethylaminopropylamide (DMAPAA) and divinylbenzene (DVB). When the molar ratio of DMAPAA to DVB was 80/20 (amino-group content : 5.1 meq/g) and the pore size (molecular mass exclusion of polysaccharide, M_<lim>) was 4000 to 10000, DMAPAA/DVB showed a high endotoxin-adsorbing activity at pH 5.0 to 9.0 and ionic strengths of mu = 0.05 to 0.4. The capacity of the adsorbent (M_<lim> : 4,000) was 390 mg of endotoxin (lipopolysaccharide purified from E.coli O111 : B4) per ml of the adsorbent in the batchwise method. The apparent dissociation constant between endotoxin and the adsorbent was 2.2 X 10-12 M.On the other hand, the adsorption of bovine serum albumin, an acidic protein, by the adsorbent increased with increase in Mlim from 4,000 to 10,000, but decreased with increase in the ionic strength (mu) from 0.05 to 0.2. As a result, DMAPAA/DVB (80/20) (M_<lim> : 4,000) selectively removed endotoxin from various acidic protein solutions at pH 7.0 and m = 0.05. The residual concentration of endotoxin in the protein solution always decreased to a concentration lower than 0.1 ng/ml and the recovery of the protein was over 97%.
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