1997 Fiscal Year Final Research Report Summary
Study of Micro Integrated Fluid System using Micro Stereo Lithography
| Project/Area Number |
07555402
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Nagoya University |
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Principal Investigator |
IKUTA Koji Nagoya Univ.Grad.Sch.Eng.Prof., 工学研究科, 教授 (90212745)
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| Project Period (FY) |
1995 – 1997
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| Keywords | biochemical IC / MIFS / Cell free synthesis of protein / Luciferase / micro reactor / DNA synthesis / 無細胞タンパク合成 / ルミノール反応 |
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| Research Abstract |
We have developed several types of biochemical IC Chips with photo detective micro reactor named "MIFS (Micro Integrated Fluid System)". The real time monitoring and controlling cell free synthesis of protein was demonstrated successfully. The photo protein of firefly so called Luciferase was synthesized. The biochemical luminescence was monitored by built-in photo diode in the micro reactor to transfer reaction status into voltage signal. Feedback control synthetic reaction was verified experimentally. The results achieved here should be key technology for future DNA and RNA synthesis without biological cell.
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