1996 Fiscal Year Final Research Report Summary
Development of High Performance Micellar Chromatography for the Determination of Serum Aluminium
| Project/Area Number |
07555564
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
工業分析化学
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| Research Institution | Tohoku University |
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Principal Investigator |
YOTHUYANAGI Takao Tohoku Univ., Fac.of Engineering, professor, 工学部, 教授 (00001199)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Makoto SHINO-TEST CORPORATION,Dep.of Research and Development, Reseacher, 研究開発部, 研究員
KANEKO Emiko Tohoku Univ., Fac.of Engineering, Associate researcher, 工学部, 助手 (00241539)
HOSHINO Hitoshi Tohoku Univ., Fac.of Engineering, Associate professor, 工学部, 助教授 (20124620)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Aluminium / Serum / Micellar Chromatography / HPLC / 8-quinolinol |
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| Research Abstract |
A new method has been developed for the determination of serum aluminium with 8-quinolinol by kinetic defferenciation mode micellar chromatography. The proposed method with a semi-micro column and fluoremetric detection enabled the determination of aluminium down to 1 ppb in human serum without preliminary deproteinization. The most remarkable point of the method is that only aluminium ion selectively responses to this system. The complex formation of aluminium in serum with 8-quinolinol was completed within a few minutes at room temperature. The reaction mixture was applied to the chromatography directly. The serum matrix and aluminium chelate were separated on a reversed-phase column with an eluent containing 17% acetonitril, 0.01 M sodium dodecyl sulfate, 1 wt% TritonX-100, and 0.1 M N,N-Bis (2-hydroxyethyl) -2-aminoethanesulfonate buffer (pH 7.0). Then aluminium complex was detected with fluorescence detector (ex 370nm, em 504nm). There was good agreemnet between the values obtained by the proposed method and those by the Zeeman graphite-furnace absorption atomic spectrophotometry.
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