1997 Fiscal Year Final Research Report Summary
Development of sensitive diagnostic methods for transmissible spongiform encephalopathies.
| Project/Area Number |
07556068
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied veterinary science
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| Research Institution | Obihiro University of Agricutture and Veterinary Medicine |
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Principal Investigator |
SHINAGAWA Morikazu Obihiro Univ., Vet.Med., Prof., 畜産学部, 教授 (00001537)
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| Co-Investigator(Kenkyū-buntansha) |
KUBO Masanori N.I.A.H.Lab.Pathol. Diag. Head Res., 家畜衛生試験場・病理診断室, 室長
YAMADA Haruo Obihiro Univ., Vet.Med., Prof., 畜産学部, 教授 (20003113)
HIRUOKA Hidefumi Obihiro Univ., Vet.Med., Ass.Prof., 畜産学部, 助教授 (60238665)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Transmissible spongiform encephalopathies / Preclinical diagnosis / ELISA |
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| Research Abstract |
In order to increase the sensitivity for detection of prion protein, the method to prepare samples from tissues for Western blotting was reformed. In mouse scrapie model, prion protein could be detected in the samples prepared from the spleen 1 week after intraperitoneal infection by the new method. It is to be desirable that more convenient method than Western blotting to examine many samples for the presence of prion protein. We developed an ELISA whose sensitivity was about two times higher than that of Western blotting using mouse model. We first confirmed that this ELISA method could be used for sheep scrapie samples, then applied the ELISA method for near 300 sheep samples obtained from slaughter house in Hokkaido.
We are developing the method for preparing samples to detect prion protein in contaminated collagen. We found the ways to reduce viscosity of collagen solution by enzymes and to concentrate prion from large volume of the enzyme treated collagen solution.
We also examined polymorphisms of sheep prion gene in relation to sheep with scrapie, expression of prion protein in various sheep organs, and polymorphisms of sheep apolipoprotein E gene as a risk factor of scrapie.
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[Publications] Ikeda, T., Horiuchi, M., Ishiguro, N., Muramatsu, Y., Kai-Uwe, G.D., and Shinagawa, M.: "Amino acid polymorphisms of PrP with reference to onset of scrapie in Suffolk and Corriedale sheep in Japan." J.Gen. Virol.76. 2577-2581 (1995)
Description
「研究成果報告書概要(欧文)」より
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