1997 Fiscal Year Final Research Report Summary
IDENTIFICATION OF LIFE CYCLE AND ESTABLISHMENT OF THE METHOD FOR DlAGNOSIS,PREVENTION AND TREATMENT OF NEOSPORA CANINUM
| Project/Area Number |
07556070
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied veterinary science
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| Research Institution | HOKKAIDO UNIVERSITY (1997)
Tottori University (1995-1996) |
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Principal Investigator |
UMEMURA Takashi HOKKAIDO UNIV., GRADUATE SCH.OF VET.MED., PROF., 大学院・獣医学研究科, 教授 (00151936)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMURA Kameo NAT.INST.ANIM.HEALTH,RES.MANAGER, 家畜衛生試験場, 室長
OONAGA Hiroshi NIPPON INST.BIOL.SCI.RES.VlCE-DIRECTOR, 次長 (80072461)
HARITANI Makoto NAT.INST.ANIM.HEALTH,SENIOR RES., 家畜衛生試験場, 主任研究官
OKUDA Koukenn VET.DIAGNST.LAB., OKAYAMA PREF., SENIOR RES., 家畜病性鑑定所, 専門研究員
TANIYAMA Hiroyuki RAKUNO GAKUEN UNIV., DEPT.OF VET.PATHOL., ASSOC.PROF., 酪農学部, 助教授 (90133800)
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| Project Period (FY) |
1995 – 1997
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| Keywords | PROTOZOA / NEOSPORA CANINUM / NEOSPOROSIS / BOVINE ABORTION / MYOSITIS / ENCEPHALITIS |
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| Research Abstract |
1.Study on the incidence of neo sporosis in Japan : the disease occurred in 24 prefectures so far and distributed widely throughout the country. The earliest occurrence of the disease was in 1985, and many cattle imported from USA had the antibody against the protozoa. Shelty dogs and dogs kept with cattle frequently had the antibody. Based on these results, a circulation of the protozoa among dogs and cattle was suspected and we recommended farmers not to keep dogs with cattle.
2.Isolation of neospora : weisolated 8 strains of neospora from aborted fetuses or congenitally-infected newborn calves in Japan. Ribosomal DNA of our isolates was identical with those isolaed in USA.Antigen plates for fluorescent antibody test and latex beads ELISA kit were prepared using our isolales.
3.Study on the mode of transmission : antibody against the protozoa was sought in various animals (dogs, cats, mice, pigeons and racoons) habitaing near the affected cattle, and none of them except dogs had the antibody. Nude mice experimentally infected with the protozoa showed radiculoneuritis and a proliferation of the parasites in the digestive tract. These findings suggested the a possible vertical transmission of neospora by the feces excreted from infected animals.
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[Publications] Sawada, M., Park, C.-H., Kondo, H., Morita, T., Shimada, A., Yamane, I.and Umemura, T.: "Serological survey of antibody to Neospora caninum in Japanese dogs." Journal of Veterinary Medical Science. (in press). (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamane, I., Shibahara, T., Kokuho, T., Shimura, K., Hamaoka, R., Haritani, M., Conrad, P.A., Park, C.-H., Sawada, M.and Umemura: "T.An improved isolation technique of bovine Neospora sp." Journal of Veterinary Diagnostic Investigation. (in press). (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamane, I., Kokuho, T., Shimura, K., Eto, M., Shibahara, T., Haritani, M., Ouchi, Y., Sverlow, K.and Conrad, P.A: "In vitro isolation and characterisation of a bovine Neospora species in Japan." Reserch in Veterinary Sciences. 63. 77-80 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Sawada, M., Park, C.-H., Morita, T., Shimada, A., Umemura, T.and Haritani, M.: "Pathological findings of nude mice inoculated with bovine Neospora." Journal of Veterinary Medical Science. 59. 947-948 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamane, I., Kokubo, T., Shimura, K., Eto, M., Haritani, M., Ouchi, Y., Sverlow, K.W.and Conrad, P.A.: "In vitro isolation of a bovine Neospora in Japan." Veterinary Record. 138. 652 (1996)
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[Publications] Okuda, K., Hlrata, Y., Fukutomi, T., Fujiwara, M., Shimada, A.and Umemura, T.: "Neospora induced hind limb paralysis in a 4-week-old Holstein calf." Journal of Japanese Veterinary Medical Association. 48 (in Japanese with English summary).544-546 (1995)
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「研究成果報告書概要(欧文)」より
