1996 Fiscal Year Final Research Report Summary
Establishement of genetic resource of plants against multispectrum disease by using peptide sequences
| Project/Area Number |
07556071
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
生物資源科学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NUMBA Shigetou Graduate School of Agriculture and Agricultural Life Science, Professor, 大学院・農学生命科学研究科, 教授 (50189221)
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| Co-Investigator(Kenkyū-buntansha) |
魯 暁云 日本学術振興会, 特別研究員
TANAKA Yoshikazu Suntory Ltd., Plant Biotechnology Laboratory, Researcher, 基礎研究所, 研究員
ASHIKARI Toshihiko Suntory Ltd., Plant Biotechnology Laboratory, Section Manager, 基礎研究所, 主任研究員
LU Xiaoyun Japan Society pf the Promotion of Science, Research fellow
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| Project Period (FY) |
1995 – 1996
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| Keywords | Trichovirus genus / Potyvirus family / citrus tatter leaf virus / potato virus Y / azuki bean mosaic virus / tobacco etch virus / multispectrum |
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| Research Abstract |
In this project, we have developed our work as follows :
We have established the technique to add several preventing functions such as de-integument, copying, moving between cells or a body, and maturity of virus protein. Further, we have tried to produce a plant which has the newest resistant properties beyond genus of virus, and establish genetic resource of plants against multispectrum disease.
First, we have produced plants which have potato virus Y,azuki bean mosaic virus, tobacco etch virus, citrus tatter leaf virus, and movement protein and coat protein of potato virus T.Then we have analyzed the functions of each protein. Proceedingly, we have produced full length of cDNA clones of citrus tatter leaf virus genome, composed infectious transcribing RNA and established a system to infect plants by inoculation. Under these results, we have introduced a mutation to the cDNA,investigated influences to the function of the protein, and got a lot of informations. Therefore, it would be possible to get significant knowledges to apply various protein to plants and produce plants which have multispectrum resistant properties in the future.
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[Publications] Sato,M.,Nanba,S.,Katsuhara,M.,Kawakita,H.,Mitsuhashi,W.,Kawabe,S.: "PCR (Polymerase Chain Reaction) Detection of Phytoplasma in Phloem Sap Collected by Laser Stylectomy" 日本植物病理学会報. 62・2. 177-180 (1996)
Description
「研究成果報告書概要(和文)」より
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[Publications] Sato, M., Namba, S., Katsuhama, M., Kawakita, H., Mitsuhashi, W & Kawabe, S.: "PCR (Polymerase Chain Reaction) Detection of Phytoplasma in Phloem Sap Collected By Laser Stylectomy" Annals of the Phytopathological Society of Japan. Vol. 62.2. 177-180 (1996)
Description
「研究成果報告書概要(欧文)」より
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