1997 Fiscal Year Final Research Report Summary
Experimental Pathological Evaluation on Laboratory Suncus for Tonsillar Research
| Project/Area Number |
07557026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Experimental pathology
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| Research Institution | Kansai College of Oriental Medicine |
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Principal Investigator |
KIMURA Michio Kansai College of Oriental Medicine, Professor, 鍼灸学科, 教授 (50111745)
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| Co-Investigator(Kenkyū-buntansha) |
KUKI Kiyonori Wakayama Medical College, School of Medicine, Lecturer, 医学部, 講師 (40169975)
TOHYA Kazuo Kannsai College of Oriental Medicine, Associate Professor, 鍼灸学科, 助教授 (90183491)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Tonsillar Immunity / Suncus T Cell / Suncus B Cell / Monoclonal Antibody / Palatine Tonsil / Tubal Tonsil / Animal Model / Tonsil Research |
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| Research Abstract |
Clinico-pathologically, human tonsils have been marked as an important iducer site of mucosal immunity. Neverthless, the tonnsillar laboratory investigations are very little because the present conventional laboratory animals ; mice, rats and guinea pigs lack tonsils.
Under such conditions, a new laboratory animal, Suncus (Suncus Murinus) was selected in this study. The animal has a pair of palatine and tubal tonsils. The individual tonsils consisted of crypt-like lymphoepithelialsymbiosis and single lymph nodules. Their crypt-epithelia contained M cells in the luminal sites. The postcapillary venules and efferent lymphatics localized in the marginal regons. Immunohistochemical preparations stained by our developed anti-Suncus T and B cell monoclonal antibodies revealed that T cells occupied the parafollicular zone and B ells located within the follicles. In the intra-tympanic cavity horseradish peroxidase (HRP) sensitization, numerous HRP-antibody positive B cells were detected not only in the tubal tonsil but rarely in their regional lymph node, spleen and palatine tonsil. Although it is unclear whether the HRP-antibody consists of IgG class or IgA,the presence of M cells and the mesangial HRP-antibody deposition pressume that the antibody partially contain IgA ones. The present study supports that the suncus is worthy for understanding tonsillar regional immunity. We are trying to develope anti-Suncus IgA antibody monoclonal antibody. The IgA monoclonal antibody will bring us further significant information in the tonsil pathology.
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[Publications] Hashimoto, K., Tsujimura, T., Moriyama, Y., Yamatodani, A., Kimura, M., Tohya, K., Morimoto., Kitayama, H., Kanamura, Y.and Kitamura, Y.: "Transforming and differetiation-inducing potential of constitutively activated c-kit mutant genes in the IC-2 murine interleukin-3 dependent mast cell line." Amer. J.Pathol.148. 189-190 (1996)
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