1996 Fiscal Year Final Research Report Summary
ヒトゲノム反復配列の不安定性にもとづく疾患の診断技術の開発
| Project/Area Number |
07557043
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
内科学一般
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| Research Institution | TOKYO INSTITUTE OF TECHNOLOGY |
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Principal Investigator |
ISHIKAWA Fuyuki Tokyo Institute of Technology Deaprtment of Life Science Professor, 生命理工学部, 助教授 (30184493)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Hideo Yokohama Research Center, Mitsubishi Chemical Corporation Pharmaceutical Laborat, 横浜総合研究所, 研究員
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| Project Period (FY) |
1995 – 1996
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| Keywords | telomere / telomerase / stretch PCR assay / leukemia |
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| Research Abstract |
Telomeres, the ends of chromosomes, are essential components of chromosomes for their stable maintenance.They show a highly dynamic changes, resulted from an equilibrium between the telomere shortening due to cell growth and the telomere elongation due to the telomerase activity. We measured the telomere lengths in normal peripheral blood cells and found that they are shortened as the ages of the donors increased. In leukemic cells, the telomere lengths were invariably shortened compared to those of the normal cells.We developed a novel quantitative stretch PCR assay for the neasurement of the telomerase activity. Telomerase was negarive in most of the normal blood cells. In contrast, it was highly activated in advanced stages of malignancy, such as blastic crisis of CML and relapsed casesof AML.The telomere crisis model was proposed to explain these characteristic features with malignant cells.
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[Publications] Tatematsu, K., Nakayama, J., Danbara, M., Shionoya, S., Sato, H., Omine, M., and Ishikawa, F.: "A novel quantitative "stretch PCR assay", that detects a dramatic increase in telomerase activity during the progression of myeloid leukemias." Oncogene. 13. 2265-2274 (1996)
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[Publications] Saito, T., Matsuda, Y., Suzuki, T., Hayashi, A., Yuan, X., Saito, M., Nakayama, J., Hori, T., and Ishikawa, F.: "Comparative gene mapping of the human and mouse TLP1 genes, that encode one protein component of telomerases." Genomics. 46. 46-50 (1997)
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[Publications] Nakayama, J., Tahara, H., Tahara, E., Saito, M., Ito, K., Nakamura, H., Nakanishi, T., Tahara, E., Ide, T.and Ishikawa, F.: "Telomerase activation by the catalytic component gene TRT in human primary fibroblasts and hepatocellular carcinomas." Nature genetics. 18. 65-68 (1998)
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「研究成果報告書概要(欧文)」より
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